ارزیابی تنوع صفات مورفولوژیک و فعالیت ضد میکروبی اکوتیپ‌های پونه (Mentha longifolia) جمع‌آوری شده از رویشگاه‌های استان لرستان

نوع مقاله : مقاله علمی - پژوهشی

نویسنده

استادیار، گروه کشاورزی، دانشگاه پیام نور، تهران، ایران

چکیده

به‌منظور بررسی تنوع صفات مورفولوژیکی پونه (Mentha longifolia) و تعیین فعالیت ضدباکتریایی آن علیه سه سویه باکتریKlebsiella pneumonia ،monocytogenes Listeria  و  Streptococcus agalactiaسه اکوتیپ پونه از سه رویشگاه استان لرستان شامل خرم‌آباد، الشتر و دلفان جمع‌آوری و برای صفات ارتفاع بوته، طول برگ، عرض برگ، تعداد برگ، تعداد انشعابات، تعداد گل‌آذین، طول گل‌آذین و درصد اسانس مورد تجزیه واریانس یک‌طرفه قرار گرقتند. نتایج نشان داد که تفاوت معنی‌داری بین اکوتیپ‌های مورد مطالعه از نظر طول و عرض برگ، ارتفاع گیاه و درصد اسانس وجود داشت. اکوتیپ خرم‌آباد دارای بالاترین ارتفاع گیاه، طول گل‌آذین و درصد اسانس بود. همبستگی مثبت و معنی‌داری بین صفات ارتفاع بوته و عرض برگ با درصد اسانس مشاهده شد. نتایج تجزیه رگرسیون گام‌‌به‌گام و تجزیه علیت نشان داد که ارتفاع گیاه می‌تواند مهمترین شاخص برای انتخاب لاین‌های والدینی در برنامه‌های اصلاحی باشد. با توجه به شاخص‌های حداقل غلظت مهارکنندگی باکتری (MIC) و حداقل غلظت باکتری‌کشی (MBC) نتیجه‌گیری شد که حداقل غلظت بازدارندگی و کشندگی اسانس پونه بین 2-03/0 میلی‌گرم در میلی‌لیتر بود. فعالیت ضدمیکروبی حکایت از این داشت که باکتری‌های L. monocytogenes و S.agalactiae توسط اسانس اکوتیپ دلفان و باکتری K. pneumonia توسط اسانس اکوتیپ الشتر مهار شدند و سه باکتری مورد مطالعه کمترین حساسیت را به اسانس پونه اکوتیپ خرم‌آباد داشتند.

کلیدواژه‌ها

موضوعات

مراجع

  • Abbaszadeh, B., Rezaee, M. and Layegh Haghighi, M., 2012. Investigation morphological characteristics and essential oil component of 2 ecotypes of watermint (Mentha aquatica). Journal of Medicinal Plants, 1 (41): 248-257. (In Persian).
  • Abou El-Nasr, T.H.S., Ibrahim, M.M., Aboud, K.A. and El-Enany, M.A.M., 2013. Assessment of genetic variability for three corianders (Coriandrum sativum) cultivars grown in Egypt, using morphological characters, essential oil composition and ISSR markers. World Applied Science Journal, 25 (6): 839-849.
  • Adams, R.P., 2001. Identification of essential oil components by gas chromatography and Mass Spectrometry. Allured, USA. 750p.
  • Anwar, F., Alkharfy, K.M., Rehman, N., Adam, E.H.K. and Gilani, A.H., 2017. Chemo-geographical variations in the composition of volatiles and the biological attributes of Mentha longifolia essential oils from Saudi Arabia. International Journal of Pharmacology, 13 (5): 408-424.
  • Atashgahi, Z., Ejtehadi, H. and Zare, H., 2009. Study of florists, life from and chorology of plants in the east of Dodangeh forests, Mazandaran province, Iran. Iranian Journal of Biology, 22 (2): 193-203. (In Persian)
  • Azarkish, P., Moghaddam, M., Vaezi, J., Ghasemi Pirbalouti, A. and Davarinejad, Gh., 2016. Genetic diversity of ecotypes of horse mint (Mentha longifolia) in the southwest of Iran using morphological traits. Seed and Plant Improvement Journal, 32 (3): 311-329. (In Persian)
  • Bajalan, I. and Pirbalouti, A.G., 2014. Variation in antibacterial activity and chemical compositions of essential oil from different populations of Myrtle. Industrial Crops and Products, 61: 303-307.
  • Basheer, N., Modawi, A., Elamin, H.B. and Ibrahim, H.M., 2015. A potential new isolate for streptokinase production. International Journal of Current Microbiology and Applied Sciences, 4: 380-7.
  • Behera, T.K., Gaikward, A.B., Singh, A.K. and Staub, J.E., 2008. The relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia ). Journal of the Science of Food and Agriculture, 88: 733-737.
  • Giamarellou, H. and Poulakou, G., 2009. Multidrug-resistant gram-negative infections. Drug, 69 (14): 1879-1901.
  • Glisic, S.B., Milojevic, S.Z., Dimitrijevic, S.I., Orlovic, A.M. and Skala, D.U., 2007. Antimicrobial activity of the essential oil and different fractions of Juniperus communis and a comparison with some commercial antibiotics. Journal of the Serbian Chemical Society, 72: 311-320.
  • Hadian, J., Karimi, E., Shouryabi, M., Nadjafi, F. and Kanani, M.R., 2016. Evaluation of morphological variation and path coefficient analysis of oil content of Thymus daenensis Celak populations. Plant Production Technology, 16 (1): 41-56. (In Persian)
  • Hasanpour Reihani, K., Ghasemi, R. and Sofalian, A., 2016. Study of morphological variation of Mentha longifolia The 5th National Conference on Agricultural and Sustainable Natural Resources, Tehran, Iran. (In Persian)
  • Hof, H., Nichterlein, T., Lampidis, R. and Wecke, J., 1998. Listeria dispose of many facettes. Biotest Bulletin, 6: 21-3.
  • Hosseini, S.A. and Dori, M.A., 2005. Preliminary investigation on the establishment and performance of flower of Hypericum perforatum collected from Diarrhoea and Garmabad in the Golestan province. Iranian Journal of Medicinal and Aromatic Plants, 20 (4): 397-406. (In Persian)
  • Hosseini, Z., 2016. Evaluation of morphological and phytochemical characteristics of Mentha longifolia during flowering in Fars and Khorasan Razavi province. M.Sc. Thesis. University of Torbat Heydarieh, Iran. (In Persian)
  • Jorgensen, H. 1993. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard. Wayne, PA:National Committee for Clinical Laboratory Standards Antimicrobial Susceptibility Testing. NCCLS M7-A3.
  • Kalemba, D. and Kunicka, A., 2003. Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry; 10 (10): 813-839.
  • Khanuja, S.P.S., Shasany, A.K., Srivastava, A. and Kumar, S., 2000. Assessment of genetic relationships in Mentha Euphytica, 111: 121-125.
  • Koba, K., Sanda, K., Raynaud, C.D., Nenonene, Y.A., Millet, J. and Chaumont, J.P., 2004. Antimicrobial activities of essential oils from African cymbopogon against microorganisms pathogenic in pets. Annales de Médecine Vétérinaire, 148, 202-206
  • Masomi, J., Yadegari, D. and Mozoni, Sh., 2005. More appropriate antimicrobial agents for antibiogram. Iranian Journal of Infectious Diseases and Tropical Medicine, 10 (29): 53-58.
  • Masoumipour, F., Hassanshahian, M. and Jafarinasab, T., 2018. Antimicrobial activity of combined extracts of Trachyspermum, Thymus and Pistachio against some pathogenic bacteria. Journal of Kerman University of Medical Science, 25(2): 153-63.
  • Mian, G.F., Godoy, D.T., Leal, C.A.G., Yuhara, T.Y., Costa, G.M. and Figueiredo, H.C.P., 2009. Aspects of the natural history and virulence of S.agalactiae infection in Nile tilapia. Veterinary Microbiology Journal, 136: 180-183.
  • Mohsenipour, Z. and Hassanshahian, M., 2016. Antibacterial activity of euphorbia hebecarpa alcoholic extracts against six human pathogenic bacteria in planktonic and biofilm forms. Jundishapur Journal of Microbiology, 9 (6): 1-7.
  • Moshtaghi, H. and Boniadian, M., 2008. The effect of oil of Mentha spicata on Listeria in food model. Iranian Journal of Medicinal and Aromatic Plants, 24 (3): 326-332.
  • Mousavi,M. and Hemmati, Kh., 2014. Effect of height on essential oil of Mentha longifolia L. in Chaharmahal va Bakhtiari province. Natioal Conference of Medicinal Plant, Islamic Azad University, Ayatollah Amoli Branch. Tehran. (In Persian).
  • Pourali Sheshblouki, G.H., Mardaneh, J. and Hosseinzadeh, Z., 2016. Klebsiella pneumonia infections in hospitalized patients: characterization of antibiotic cross-resistance and detection of cefepime susceptible-dose dependent strains. Journal of Fasa University of Medical Sciences, 6 (1): 52-59. (In Persian)
  • Rahimi, M.H., Aharizad, S. and Mohebalipour, N., Evaluation of genetic diversity in populations of Lemon balm (Melissa officinalis) in terms of agronomic traits, essential oil and central concentration. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 25 (2): 271-288. (In Persian)
  • Saadatjou, B., Mohammadkhani, A., Saeidi, K. and Shirmaridi, H.A., 2015. The evaluation of morphological variation and essential oil content of Salvia species ecotypes in south-west of Iran. Applied Crop Breeding, 3 (1): 125-135. (In Persian)
  • Sadeghian, I., Hassanshahian, M., Sadeghian, S. and Jamali, S., 2012. Antimicrobial effects of Quercus Brantii fruits on bacterial pathogens. Jundishapur Journal of Microbiology, 5 (3): 465-9.
  • Shahbazi, Y., 2015. Chemical composition and in vitro antibacterial activity of Mentha spicata essential oil against common food-borne pathogenic bacteria. Journal of Pathogens: 1-5.
  • Shan, B., Cai, Y.Z., Brooks, J.D. and Corke, H., 2007. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. International Journal of Food Microbiology, 117(1): 112-119.
  • Singh, R., Shushni, M.A.M. and Belkheir, A., 2015. Antibacterial and antioxidant activities of Mentha piperita. Arabian Journal of Chemistry, 8: 322-328.
  • Singh, S.P., Sharma, and Tewari, R.K., 1998. Genetic improvement of Mentha arvensis based on essential oil yield and quality traits. Journal of Herbs, Species and Medicinal Plants, 6 (2): 79-86.
  • Strycharz, S. and Shetty, K., 2002. Peroxidase activity and phenolic content in elite clonal lines of Mentha pulegium in response to polymeric dye R-478 and Agrobacterium rhizogenes. Process Biochemistry, 37 (8): 805- 12.
  • Tserennadmid, R., Tako, M., Galgoczy, L., Papp, T., Vagvolgyi, C., Gero, L. and Krisch, J., 2010. Antibacterial effect of essential oils and interaction with food components. Central European Journal of Biology, 5(5): 641-648.
  • Tsuchiya, H., Sato, M., Miyazaki, S., Fujiwara, S., Tanigaki, S., Ohyama, M., Tanaka, T. and Linuma, M. 1996. Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology, 50 (1): 27-34.
  • Vining,J., Zhang, Q., Tucker, A.O., Smith, C. and Davis, T.M., 2005. Mentha longifolia a model species for mint genetic research. Horticultural Science, 40 (5): 1225-1229.
  • Zeinali, H., Vali, E., Safaei, L. and Enteshari, Sh., 2012. Study of reproductive trait variation in Mentha Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 20 (2): 327-339. (In Persian)
  • Zinodini, A., Farshadfsr, M., Safari, H., Moradi, F. and Shirvani, H., 2013. Study of genetic relationships of some Mint species using ISSR markers. Crop Biotechnology, 3 (5): 11-21. (In Persian)

فایل ها

هم رسانی

ارجاع به این مقاله

آمار