Comparision of qualitative and quantitative activities of peroxidase in different organs of Sorbus torminalis (L.) Crantz

Iranmanesh, Yaghoub; Aliahmad Crouri, Soudabeh; Spahbodi, Kambiz; Azadfar, Davood

Comparision of qualitative and quantitative activities of peroxidase in different organs of Sorbus torminalis (L.) Crantz

Document Type : Research Paper

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Abstract

Enzymes are important factors on metabolism functions of plants. Enzyme was introduced as a suitable index in order to response to alteration of environmental factors. This research was carried out on Farim forest region on Mazandaran province of Iran by selecting 40 trees of S. torminaslis (L.) Crantz. Seeds were obtained from all of the selected trees and the seedlings were produced for each single tree separately. In order to investigate enzymatic activities in different organs, four trees with the same characteristics were selected. Sampling were done on branch and leaf of seedlings and different organs. The samples' enzymes were extracted immediately and peroxidase was quantitatively and qualitatively studied. Quantitative studies were acomplished by Spectrophotometer (in 530 NM wave length) and qualitative studies were performed by polyacrylamid gel electrophoresis (PAGE). Comparison of isoenzymatical patterns of the leaf and branch showed existence of a specific band in leaf and branch samples that shows plant ability in response to ecological alteration. Also quantitative activity of peroxidase in branch was 0.186 and in leaf was 0.085 that was statistically significant. The number of isoenzymes of branch and leaf were 15 and 12 respectively.

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