Document Type : Research Paper
Authors
1
Corresponding author, M.Sc., Agricultural Biotechnology, Research Institute of Forests and Rangelands, Tehran, I.R.Iran
2
Prof., Research Institute of Forests and Rangelands, Tehran, I.R.Iran.
3
Scientific board member of Research Institute of Forests and Rangelands, Tehran, I.R.Iran
4
Prof., Payam Noor University, Tehran, I.R.Iran
5
M.Sc., Research Institute of Forests and Rangelands, Tehran, I.R.Iran
Abstract
Moringa peregrina is one of the important species which is growing in a vast area of the Southeast part of Iran. Despite of its importance in medicinal industry and living environment, the species did not receive enough attention by now. In other words, there was not enough research on its genetic potentials. Due to various reasons, the species is under genetic erosion; therefore, in order to optimize its remarkable characteristics exploitation, its conservation through various methods is considerable. Current research, multiplication of the species through lateral buds by tissue culture, was employed in order to find a suitable culture media for the species multiplication as well as investigating its plant populations' different responses to micropropagation and several culturing media. Immature seeds of three plant populations were collected from three locations of its habitats, Chanf, Benet and Kenshki, and cultured on aseptic conditions. The seedlings containing one or two buds were transferred to the multiplication culturing media. MS and Half-MS culturing media along with four hormone combinations ( 0.5 and 1 mg/l BAP, combination of BAP and 2ip, 0.5 mg/l) were used in the study. Several characteristics such as number of branches, active buds, and branches average length were recorded. There were significant differences between the studied plant populations based on the number of branches. Whereas, there wasn't significant differences between the populations based on branches length. Chanf population had the highest value in branch production between the studied plant populations. The longest branch was also observed on the Chanf plant population.
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