Using RAPD marker for genetic diversity assessment of several Thymus  Species

Alamdary, S.B.L.; Safarnejad, A.; Nematzadeh, G.A.

doi:10.22092/ijrfpbgr.2013.3477

Using RAPD marker for genetic diversity assessment of several Thymus Species

Document Type : Research Paper

Authors

¹ M.SC, Agricultural Biotechnology, Agricultural Sciences and Natural Resources University of Sari, I.R.Iran

² Assoc. Prof., Razavi-Khorasan Agriculture and Natural Resources Center, Mashhad, I.R.Iran

³ Prof., Plant Breeding Sari University of Agricultural Sciences and Natural Resources, Sari, I.R.Iran

10.22092/ijrfpbgr.2013.3477

Abstract

Thirteen accessions of thyme medicinal plant belonging to Iranian endemic species of Thymus daenensis, T. migricus, T. pubsence and T. fedtschenkoi were studied to asses genetic variation. Genomic DNA was extracted using modified CTAB protocol. DNA was qualified using electrophoresis. Twenty primers were used for PCR analysis, of which only 8 primers showed clear bands. Out of 97 bands 61% were polymorphic. The number of amplification products per primer varied from 8 (OPP3) to 16 (DPA 9). T. daenensis (No. 8 and 10) were in the lowest genetic distance and T. migricus and T. daenensis (No. 11) were in the most genetic distance and the lowest genetic similarity. Dendrogram showed two major clusters. Group 1 was formed by T. daenensis, T. pubsence and T. migricus. Group 2 was formed by T. fedtschenkoi and T. daenensis subs daenensis (No.7). Finally investigating the genetic variation of the plant species by RAPD indicated that RAPD marker is suitable to determine the polymorphic loci and to estimate the genetic distance on the species.

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