Assessment of micropropagation and flavonoid content of hawthorn  through tissue culture

Moghimi, Zahra; Safarnezhad, Abbas

doi:10.22092/ijrfpbgr.2015.12218

Assessment of micropropagation and flavonoid content of hawthorn through tissue culture

Document Type : Research Paper

Authors

10.22092/ijrfpbgr.2015.12218

Abstract

Hawthorn(Crataegus sp.) is one of the plants, which suffers of low seed germination rate. Purposes of this research was evaluation of optimizing a medium for propagation of hawthorn and comparison of callus flavonoid, to that of leaf and fruit of the species. Media including MS, N₆ and B₅ supplemented by various plant growth regulators were used. The study was carried out based on a completely randomized design with 3 replicates. Flavonoid content was determined using a colorimetric (Aluminum Chloride) method. Results showed that mercuric chloride %0.02 for 3 min, ethanol %70 for 2 min and NaOCl for 15 min were the best treatments for sterilization. N₆ medium supplemented with 7 mg/L BA + 1.5 mg/L NAA was the best medium for callus induction. The most regeneration was observed in B₅ medium supplemented with 0.49 mg/L BA + 0.49 mg/L Kin and 0.1 mg/l IAA and MS medium supplemented with 1 mg/L BA + 0.5 mg/L IAA was the best for multiplication. Rooting was observed only in MS medium supplemented with 1 mg/L IBA with low frequency. The results indicated that flavonoid content of leaf was more than fruit and flavonoid content of callus was very low.

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