Investigation in gene transforming method to sorrel (Rumex acetosa L.)

Document Type : Research Paper

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Effective factors on gene transferring to sorrel were considered. Agrobacterium, strain LBA4404 containing pBI121 plasmid, and different concentrations and inoculation time using leaf explants were used for evaluation of the gus gene transformation. Culture of leaf segments on MS medium containing 1.5 mg/l BAP and 0.75 mg/l IAA and kanamycin  concentrations (0, 10, 25, 50 75 and 100 mg/l) showed that 25 mg/l kanamycin inhibited callus production and plant regeneration and it was determined as the effective  kanamycin level in selection medium. Effects of Agrobacterium concentration (OD= 0.2, 0.6 and 1) with different inoculation duration were studied. For this purpose leaf explants were inoculated with Agrobacterium suspension having pBI121 plasmid containing the gus gene. After co-cultivation the explants were transferred on regeneration medium containing 500 mg/l carbenicilin, 200 mg/l cefotaxim and 25 mg/l kanamycin. GUS assay showed the successful transfer of this gene in regenerated plants under OD= 0.2, 0.6 and 2 minute inoculation duration.   

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