Evaluation of genetic diversity of several accessions of Iranian Hyoscyamus niger L. based on RAPD markers

Yousefi, Mohammad Javad; Hasani, Mohammad-smaeel; Madah-aarefi, Hasan; Mohammadi-pour, Matin

Evaluation of genetic diversity of several accessions of Iranian Hyoscyamus niger L. based on RAPD markers

Document Type : Research Paper

Authors

Abstract

Genetic diversity of 19 accessions of Hyoscyamus niger L., collected from different parts of Iran, were evaluated using random amplified polymorphic DNA (RAPD) data. Total Genomic DNA was extracted from young leaves of plants and Polymerase Chain Reaction (PCR) was performed using Sixteen RAPD primers and produced a total of two hundred and eight bands, with one hundred and ninety six polymorphic bands (94.31%) between single plants of the investigated accessions. RAPD products were scored for presence (1) or absence (0) of each amplicon evaluated using Binary method and subsequently genetic similarity was calculated by employing Dice index and cluster analysis was carried out according to UPGMA algorithm using NTSYS-pc software. Accession of Isfahan showed maximum difference with other accessions and classified in separate group and maximum similarity was observed between accessions of Roodbar- 2 and Siahkal- 1 with 0.80 similarity. Results showed a moderate level of genetic diversity among investigated accessions. The results also indicated that RAPD approach seemed to be best-suited for fingerprinting and assessing genetic diversity among H. niger accessions with high accuracy.

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