Effects of medium on somatic embryogenesis of Olea europea L. (cv. Kroneiki)

Peyvandi, Maryam; Farahzadi, Helala Nemat; Arbabiyan, Sedigheh; Hoseynimazinani, Mahdi

Effects of medium on somatic embryogenesis of Olea europea L. (cv. Kroneiki)

Document Type : Research Paper

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Abstract

Effects of subculture media on somatic embryogenesis and rhizogenesis in different parts of mature embryo of Olea europaea L. (cv. Kroneiki) was studied. Radicle and proximal part of cotyledon were cultured in OMc medium containing or IBA (5 mg/l) and 2ip (0.5 mg/l). twenty one days old calli were subcultured on OMc or OM media without any hormones. Cultures were maintained in darkness. Differentiation in the calli depended on kind of explants and subculture media. After the third subculture calli of radicle and proximal part of cotyledon had the potential of somatic embryogenesis. In both media, the potentiality of embryogenesis in calli from radicle was more than calli from proximal. In addition, somatic embryogenesis in OM medium showed significant increase. After the second subculture rhizogenesis occurred in both explants and different subculture media. Differences between rhizogenesis were significant. In both subculture media the potentiality of rhizogenesis in calli from proximal part of cotyledon was much more than calli of radicle and in the both explants rhizogenesis in OM medium were more than OMc medium.

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