Variation in seed storage proteins of two Moringa species (M. oleifera and Moringa peregrina)

Asadi Corom, Fereshteh; Mirzaee-nodoushan, Hossein; Emam, Mitra; Bakhshi-khaniki, Gholamreza

Variation in seed storage proteins of two Moringa species (M. oleifera and Moringa peregrina)

Document Type : Research Paper

Authors

Abstract

Several species of Moringa, growing in tropic and sub-tropic regions of Iran, are gaining commercial value for their application in medicine, food industry, and , environmental aspects. Seed storage proteins were extracted from two species of the genus named, M. oleifera and M. peregrina. Protein profile of several plant populations of the species was obtained using SDS-PAGE, in which the protein bands were clearly separated. The bands were scored 0 or 1 for absence or presence of the single bands, respectively. The total distances of the present bands of the profile of each genotype from the beginning point on the gel were summed to get the cumulative distances as a characteristic of each profile. Cluster analysis was performed on the data matrix using JMP software. The dendrogram produced by the software was used to classify the studied genotypes. Remarkable differences were observed between the two species based on the protein profiles, suggesting that the technique can be used to classify the populations of the species. Remarkable variation was observed between the studied samples of the indigenous species, M. peregrina. In other words, the results indicated that the technique is feasible to be used to study genetic variation between the populations of the Moringa species, particularly the indigenous species.

Keywords