Effects of kinetin, 2, 4-D and TDZ on somatic embryogenesis of Eucalyptus saligna

Document Type : Research Paper

Authors

Abstract

Eucalyptus saligna with several characteristics such as medicinal and industrial uses and fast growing feature could be considered as an appropriate case to be used in countries with low forest covers such as Iran. Although reforestation by Eucalyptus species is very important, but production difficulties by conventional methods, caused in vitro culture methods to be considerable. Somatic embryogenesis is an effective tool for reforestation with improved tree species. The method is more economical than other cloning methods. Somatic embryogenesis on E. saligna was studied in this work, for the first time. Four kinds of explants, root, stems, leaves and hypocotyls of seedlings, were grown at in vitro conditions on MS medium with 13 hormone treatments. The best explants was root and the best hormone treatment was 2 mg/l kinetin with 2 mg/l 2,4-D. In general, the somatic embryogenesis results of this study were more effective than previous studies on Eucalyptus species. The embryogenic calluses were transferred to three MS mediums supplemented with different ABA concentrations to reach to maturation. Treatment with 20 mg/l ABA showed three stages of globular, heart shape and torpedo. 

Keywords

Files

Share

How to cite

Statistics