Genetic diversity assessment of Quercus infectoria and Q. libani populations in North-Zagros forests based on ISSR and IRAP markers

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Eighteen inter-simple sequence repeats (ISSR) and 10 inter-retrotransposal amplified polymorphism (IRAP) markers were applied to investigate the genetic diversity of 150 natural gall oak (Quercus infectoria) and 109 Lebanon oak (Q. libani) individuals in North-Zagros forests. Among the Q. infectoria populations, ISSR and IRAP primers amplified 202 and 136 bands, respectively, of which 194 and 135 bands were polymorphic. While, the primers amplified 178 and 134 polymorphic bands from a total of 187 and 137 amplicones in Q. libani genotypes, respectively. High level of genetic diversity at the specific level was revealed in both species. Analysis of molecular variance shawed that a major proportion of total genetic diversity belonged to within populations. In both species, the Nie’s gene diversity index (h) was higher for ISSR than IRAP primers. No significant genetic relationship was detected among genetic distance of the populations and their geographical distribution, demonstrating the genetic relationships among isolated Q. infectoria and Q. libani populations via gene flow. Cluster analysis grouped the populations of both species into distinct groups, showing efficiency of studied markers to assessment of genetic polymorphism of oaks natural populations.

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