Investigation of effective factors on sumac In vitro propagation

Poordad, Behrooz; Safarnezhad, Abbas; Ebrahimi, Mohammad Ali; Bakhshi Khaniki, Gholamreza

doi:10.22092/ijrfpbgr.2014.7663

Investigation of effective factors on sumac In vitro propagation

Document Type : Research Paper

Authors

10.22092/ijrfpbgr.2014.7663

Abstract

Sumac belongs to genus of Rhus (Anacardiaceae). Seed germination in the species is very low and it is naturally propagated through tillering. The best media for In vitro regeneration and rooting of sumac was investigated in this research. Buds were sterilized and transferred to callus induction media. Callus induction was initiated on MS basal media supplemented with IAA, BA, BAP, TDZ, IBA. Results indicated that the most callus induction and regeneration occured on MS supplemented with 1 mgl^-1 BA + 0.5 mgl^-1 IAA. Half, quarter and full strength MS medium supplemented with IAA, NAA, IBA were used for rooting. Results showed significant differences between the rooting treatments. The most rooting percentage was observed on full strength MS medium supplemented with 1 mg/l IBA. The best treatment for rooting was 1 mg/l BA + 0.5 mg/l IAA. There were significant differences between IBA concentration for length and number of root. With 5 mg/l IBA concentration, number of root increased and root length decreased. Also, there were significant differences between liquid and solid media for rooting, so that the highest rooting percentage (44.83%) was observed on solid media.

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