Micropropagation of Lonicera nummulariifolia Jaub. & Spach

Document Type : Research Paper

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Abstract

Lonicera nummulariifolia Jaub. & Spach, is a deciduous shrub growing in Zagros region of Iran as a companion species with oak, especially in Sepidan county, Fars Province, Iran. However, there isn't enough information about its propagation and different aspects of its growth. This study was carried out to develop in vitro culture techniques for rapid and mass propagation of the species. Shoot tips and nodal segments of seedlings were used as explants. Murashige and Skoog (MS) medium containing 0.1 mgl-1 BA was optimum for shoot multiplication. Nodal segments cultured on Driver and Kuniyuki walnut (DKW) medium containing 0.4, 0.7 and 1.0 mgl-1 BA, 30 gl-1 sucrose and solidified with
 8.0 gl-1 Difco Bacto agar in comparison with MS medium didn't show any significant differences on measured growth indices. The best procedure for in vitro rooting consisted of two phases: root induction and root development. For root induction the most efficient method was utilization of MS medium (half strength macronutrients) solidified with 8.0 gl-1 Difco Bacto agar, containing 30 gl-1 sucrose and 25.0 mgl-1 IBA in dark conditions for 60 hours. For root development, shoots were transferred from root induction medium to hormone free medium in lighted conditions.­ Using this method, about 46.7 percent of the shoots rooted.  After root development, plantlets were transferred to pots containing a mixture of peat, sand and vermiculite (1:1:1) and transferred to greenhouse.

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 19, Issue 1 - Serial Number 1
September 2011
Pages 71-84

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