In vitro somatic propagation of Ziziphus spina christti (L.) Willd via indirect regeneration from leaf explants

Ahmadi, Elahe; Hosseini_nasr, Seyed Mohammad; Jalilvand, Hamid; Salehian Aghblaq, Hamed

doi:10.22092/ijrfpbgr.2012.6626

In vitro somatic propagation of Ziziphus spina christti (L.) Willd via indirect regeneration from leaf explants

Document Type : Research Paper

Authors

10.22092/ijrfpbgr.2012.6626

Abstract

Regarding cross-pollination and a wide range of genetic variation in Ziziphus spina christti (L.) populations, vegetative propagation through tissue culture techniques is necessary to reproduce the elite single plants. In this study effects of different levels of growth regulators on callogenesis and regeneration capabilities of callus of leaf explants of the species were evaluated. Leaf explants of aseptic seedlings were cultured on MS medium containing 2, 4-D, NAA, TDZ and BAP levels (0.5, 1 and 2 mg/l). MS medium with different levels of BAP, TDZ and Kin (1, 3 and 5 mg / liter) was used for regeneration. Rooting process was done on 1/2MS medium with different levels of IBA and NAA levels (0.5, 1 and 2 mg /l). Results showed that the medium containing 2, 4-D and TDZ at concentrations of 0.5 and 1 mg/l (x=10) had the highest rate of callus induction. Kin treatments (5 mg/l) and TDZ (1 mg/l) with averages of 8.84 and 8.95 had the highest rate of shoot production, but the shoot produced by TDZ treatments had an unusual shape, whereas, Kin treatments, produced normal shoots. Average rooting on IBA was higher than NAA treatments on all concentrations. One mg/l IBA showed the highest rate of rooting capability compared to the other treatments.

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