Study of phylogenetic relationships of some Vicia species using storage proteins and RAPD markers

Document Type : Research Paper

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In order to evaluate phylogenetic relationships among some Vicia species, subspecies and ecotypes from Iran, 17 and 14 entries were studied using seed storage proteins electrophoresis and RAPDs markers, respectively. Total seed storage proteins were extracted from the collected seeds and DNA was extracted from leaf samples of seedlings. Nei and Shanon genetic diversity indices didn't show considerable difference between RAPD and storage protein data. While the PIC and MI indices for protein markers were greater than those of RAPD markers. In the phylogeny tree based on protein markers, the subspecies and ecotypes of sativa species were classified in the same group. In RAPD analysis, the subspecies and ecotypes were classified in a larger group along with other species. Ecotypes of V. panonica and V. cracca were classified in another group. Constructing the phylogenetic tree using incorporated RAPD and protein markers produced better results than separated ones. Also, classifying the entries using PCoA method was in correspondence to the classifying by phylogenetic trees. The distance matrices from RAPD and protein data were compared using Mantel test and were not observed any significant correlation between the two data sets.

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