Revision of Iranian Carpinus species using of molecular markers( nrDNA ITS and trnH-psbA)

Carpinus Genus is an important element in the Hyrcanian forest. There are some ambiguities and disagreements among botanist about the genus. In the current research, useful molecular markers were used for revision of the genus. We used DNA barcode nrDNA ITS and trnH-psbA and compared the performance of the two markers. CTAB method was used in order to extracting of leaves genomic DNA. PCR product sequencing and alignment and phylogenetic analyses of the dataset were conducted according to maximum parsimony (MP) method by using PAUP* A software. Results indicated that nrDNA ITS is more efficient than trnH-psbA for separation of the species. The surveyed results show that different species of the genus are placed in two separate claudes. Before that C. Betulus form Arasbaran was assumed same and similar to C. betulus from Hyrcanianforest but the current finding showed that it was completely isolated and placed in the separate claude. Furthermore, Carpinus orientalis and Carpinus schuschaensis are Synonymous. Finally, we proposed that C. betulus from Arasbaran should be considered by botanists.