Investigation of karyological characteristics in several populations of Aegilops biuncialis

Document Type : Research Paper

Authors

1 M.Sc., Faculty of Agricultre, University of Mohaghegh Ardabili, Ardabil, I.R.Iran

2 Department of Agronomy and Plant Breeding, Islamic Azad University, Ardabil Branch, Ardabil, I.R.Iran

Abstract

Aegilops species are one of the most important wild relatives of wheat and the richest genetic resources in wheat breeding. Ae. biuncialis is a tetraploid species with 28 chromosomes derived from diploid ancestors of Ae. umbellulata and Ae. comosa. The aim of this study was investigation of karyological characteristics of ten populations of Aegilops biuncialis. The karyogram was prepared from at least 12 plants of each population using aceto-iron-hematoxilin staining, and chromosomal characteristics including length of long and short arms, total chromosome length, arm ratio index and relative length were measured. Result showed that there were three pairs of satellited chromosomes with a secondary constriction in the chromosome complement of this species. Significant differences were observed among the populations for the relative length of all chromosomes except for chromosomes 10 and 13 and for arm ratio index of chromosomes 1, 2, 3, 6, 10 and 13. According to the Stebbin's classes of karyotype asymmetry, Varzqan, Ardabil and TN-01-293 populations allocated to 2A and other populations to 3A categories which show the studied populations have symmetric karyotypes. Clustering of the populations based on two first principle components and cluster analysis separated them to four distinct groups followed from their geographical distances

Keywords

Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 20, Issue 2 - Serial Number 2
October 2013
Pages 202-212

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