Study of some Artemisia L. species based on ecological, micro-morphological, and molecular evidence in rangelands of Golestan province, Iran

Document Type : Research Paper

Authors

1 M.Sc. Graduate, Department of Range and Watershed Management, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad, Iran

2 Assistant professor, Department of Range and Watershed Management, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad, Iran

3 Associate professor, Department of Range and Watershed Management, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad, Iran

4 Ph.D. Graduate, Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran

10.22092/ijrfpbgr.2023.361041.1431

Abstract

Background and objectives:
A study of indicator species to manage rangeland ecosystems and preserve the vegetation of natural areas in dry regions is necessary. The knowledge of the ecological requirements of the range species is important for planning and managing plant communities and protecting and exploiting their ecosystems. Golestan province, with its particular geographical position, is the habitat of many plant species. By identifying the factors affecting the growth and adaptation of these species, it is possible to avoid spending money and wasting time planning to improve and restore pastures. One of the valuable genera that grow in rangelands is Artemisia L., which is distributed in arid and semi-arid regions of the country and plays an essential role in soil protection, fodder supply for livestock and medicinal value.
Methodology:
In this study, 12 populations of four species of the genus Artemisia in the rangeland area in the north of Gonbad Kavos in Golestan province, Iran, were studied based on ecological, micro-morphological, and molecular evidence. The ecological studies were carried out using climate data and soil characteristics of the species' habitat. Also, the morphology of the species' pollen grain was investigated using a scanning electron microscope (SEM). The molecular relationships of four species (A. annua, A. sieberi, A. aucheri, and A. fragrans) were investigated using nuclear nrDNA ITS and chloroplast rpl32-trnL(UAG) sequences and their combinations.
Results:
The results of the ecological studies showed that many of the Artemisia species were grown in dry climates and in silty and silty loam soils. EC measurements indicated that the soils of area were saline and had poor organic matter. According to the results, many pollen grains of the species were medium-sized and tricolporate. In all studied species, according to the P/E ratio, the outline of the pollen grains, those seen in the polar view were circular, triangular, and equatorial with an elliptical outline and their exine decorations were echinate. The results of molecular studies indicated that all the species formed a monophyletic group with high support (PP=1.00, ML BS=100, MP BS=100). The combined analysis of data showed more supported relationships than the analyses of a part. The results of the cluster tree showed two separate clades. The annual species A. annua was placed in clade 1 and the rest of the studied species with high support (PP=0.88, ML BS=90, MP BS=94) were placed in clude 2.
Conclusion:
The results of current research showed that the ecological, micro-morphological characteristics 
and molecular data were useful in the distinction between the Artemisia species. Such a study allows us to select suitable and adapted genes for the environment and transfer them to the crop species to produce stress-tolerant cultivars. Artemisia is widely distributed in Iran due to its ecological adaptation and high adaptation intensity in natural conditions. Unfortunately, the change in land use and the transformation of natural areas into agricultural lands have caused to shrink the steppic areas and led to limitation of the species formation and diversity. Examining the kinship relationships between species and the evolution of traits can be used to determine the probability of success in interspecies crosses. Therefore, the obtained information can help maintain and balance the Artemisia habitat.

Keywords

Main Subjects

References

  • Ahadi Dolatsara, E., Salami, S. A., Shokrpour, M., Naghavi, M.R. and Soorni, A., 2015. Evaluation of chloroplast DNA diversity and phylogenetic relationship among 28 Iranian artemisia species. Iranian Journal of Horticultural Sciences, 45 (4): 401- 415. (In Persian).

    • Bagheri, R., Kharazmi, H. and Jaberi Ansari, S. 2013. Determining the ecological needs of Artemisia persica boiss in its natural habitats in Kerman. Plant and Ecosystem, 8: (33-1). 80 -90. (In Persian).
    • Chamani, A., Heshmati, Gh. and Karimian, V., 2015. Assessing soil surface indicators in shrubland different patches (Case Study: Goob Gogjeh rangeland of Golestan province). Environmental Erosion Research, 4 (16): 1-11.
    • Chehregani Rad, A., Atri, M. and Yousefi S., 2011. Study of intraspecific diversity of Artemisia incana (L.) Druce in east Azerbaijan. Journal of cell & tissue, 2 (3): 245 - 256. (In Persian).
    • Dolatyari, A., Vallès, J., Naghavi, M.R. and Shahzadeh Fazeli, S.A., 2013. Karyological data of 47 accessions of 28 Artemisia (Asteraceae, Anthemideae) species from Iran, with first new reports for Iranian populations and first absolute counts in three species. Plant Systematics and Evolution, 299 (1): 1503–1518.
    • Ehrendorfer, F., 1980. Polyploidy & distribution. In: Polyploidy: Biological Relevance (Lewis, W.H., ed.). Plenum Press, New York, pp. 45-60.
    • Erdtman, G. 1943: An Introduction to Pollen Analysis.Waltham Mass., 239 pp.
    • Farsi, M. and Zolali, J., 2004. Principle of Plant biotechnology. Ferdowsi Mashhad University Press, 495 pp. Mashad, Iran. (In Persian).
    • Gavili Kilaneh, E. and Vahabi, M.R., 2012. The effect of some soil characteristics on range vegetation distribution in central zagros, Iran. Water and soil science (Journal Science Technology Agriculture and Natural Resources), 16 (59): 245-258.
    • Ghahreman, A., Noorbakhsh, S., Mehdigholi, K. and Attar, F., 2007. Pollen morphology of Artemisia L. (Asteraceae) in Iran. The Iranian Journal of Botany. 13 (1): 21-29.
    • Gholami, F., Dianati Tilak, G.A. and Behtari, B., 2011. Study of allelopathic effect of Artemisia herbaalba Asso. on seed germination and seedling growth of Onobrychis sativa L. and Medicago sativa L. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 19 (1): 181-191. (In Persian).
    • Halbritter H, Ulrich S, Grímsson F, Weber M, Zetter R, Hesse M, Buchner R, Svojtka M, Frosch-Radivo A. (2018) Illustrated Pollen Terminology. Illustrated Pollen Terminology. Springer International Publishing, 487 pp.
    • Hayat, M.Q., Ashraf, M., Khan, M.A., Mahmood, T., Ahmad, M. and Jabeen, S.H., 2009. Phylogeny of Artemisia L.: Recent developments. African Journal of Biotechnology. 8(11): 2423-2428.
    • Hussain, A., Potter, D., Kim, S., Hayat, M.Q. and Bokhari, S.A.I., 2019. Molecular phylogeny of Artemisia (Asteraceae-Anthemideae) with emphasis on undescribed taxa from Gilgit-Baltistan (Pakistan) based on nrDNA (ITS and ETS) and cpDNA (psbA-trnH) sequences. Plant Ecology and Evolution, 152(3): 507-520
    • Jackson, R.C., 1976. Evolutionary and systematic significance of polyploidy. Annual Review of Ecology and Systematics. 7 (1): 209-234.
    • Jafari, M., Zare Chahooki, M.A., Rahimzadeh, N. and Shafihzade Nasrabadani, M., 2008. Comparison of litter quality and its effect on habitat soil of three range species in Vardavard region. Range Journal, 12 (1): 1-10. (In Persian).
    • Jafari-Parizi, , Afsharzadeh, S., Akafi, H. and Abbasi, S., 2014. Ecological study of Artemisia aucheri communities in three rangelands of Isfahan province. Journal of Plant Ecosystem Conservation, 2 (4):79-94. (In Persian).
    • Jalili, A., 2016. Ecology, Evolution and Biogeography of Artemisia L. Research institute of Forest and Rangeland. Tehran, Iran. (In Persian).
    • Kamali, N., Jalili, A., Ashouri, P. and Khodagholi, M., 2021. Artemisia, the largest rangeland ecosystem in Iran. Iran nature, 6 (5): 35 – 43. (In Persian).
    • Khorami Pour, , Mafi Gholami, R. and Jahani, A., 2019. An investigation on the possibility of heavy metal (Pb and Ni) phytoremediation from plant Artemisia Sieberi In Mohammadabad Waste Disposal Site In Ghazvin. Journal of Environmental Science and Technology, 21 (89): 93 – 105. (In Persian).
    • Lee, J.H., Lee, J.W., sung, J.S., bang, K.H. and Moon, S.G., 2009. Molecular Authentication of 21 Korean Artemisia Species (Compositae) by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Based on trnL–F Region of Chloroplast DNA. Biological and Pharmaceutical Bulletin, 32, 1912-1916.
    • Lu, L. L., Jiao, B. H., Qin, F., Xie, G., Lu, K. Q., Li, J. F., and Wang, Y. F., 2022. Artemisia pollen dataset for exploring the potential ecological indicators in deep time. Earth System Science Data, 14(9): 3961-3995.
    • Magurran, A.E., 1988. Ecological diversity and its measurement, Princeton University Press, Princeton. New Jersey, 179 p.
    • Mahmood, T., Hassan, N., Nazar, N. and Naveed, I., 2011. Phylogenetic analysis of different Artemisia species based on chloroplast gene rps11. Archives of Biological Sciences. 63: 661-665.
    • McArthur, E.D, Pope, C.L., and Freeman, D.C., 1981. Chromosomal studies of subgenus Tridentatae of Artemisia: evidence for autopolyploidy. American Journal of Botany, 68 (1): 589-605.
    • Mirhaji, S.T., Jalili, A., Jafari, M., Akbarzadeh, M. and Farzaneh, Z., 2001. Ecological comparison of Artemisia species in Semnan Province. Pajouhesh-Va-Sazandegi. 14 (3): 95 - 102. (In Persian).
    • Moghadam, M.R., 2009. Range and Range management. University of Tehran Pub., Tehran, 470 p. (In Persian).
    • Molaei, M. and Ghorbani, A., 2021. Effects of ecological factors on the distribution of Artemisia melanolepis and Artemisia aucheri in southeast of Sabalan, Iran. Ecopersia, 9 (1): 95-104.
    • Mozaffarian, V., 1988. Botanical Study of Artemisia L. in Iran. Thesis for master degree of science, - Faculty of Science, Tehran University. (In Persian).
    • Noorbakhsh, S.N., Ghahreman, A., Attar, A. and Mahdigholi, K., 2008. Leaf anatomy of Artemisia (Asreraceae) in Iran and ITS taxonomic implications. Iranian Journal of Botany, 14 (1). 54-69
    • Parsa, A., 1978. Flora of Iran. Ministry of Science and Higher Education of Iran. 500 pages.
    • Posada, D. and Buckley, T.R., 2004. Model selection and model averaging in phylogenetics: Advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology, 53: 793–808.
    • Ronquist, F. and Huelsenbeck, J., 2003. MrBayes 3: Bayesian phylogenetic inference under mixed Bioinformatics, 19: 754–755.
    • SeyedRahmani, R., Naghavi, M.R., Mohammadi, V. and Ranjbar, M., 2014. Relationship between expression of main MEP pathway genes and monoterpenes contents in Artemisia annua. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 22 (2): 192-200. (In Persian).
    • Shaw, J. et al., 2007. Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in Angiosperms: the tortoise and the hare III. – American Journal of Botany, 94 (1): 275–288.
    • Swofford, D. L., 2002. PAUP: Phylogenetic analysis using parsimony (and other methods), ver. 4.0b10. -Sinauer Associates, Sunderland.
    • Ter Braak, C.J.F. and Prentice, I.C., 1988. A theory of gradient analysis. Advances in Ecological Research, 18 (1): 271-317.
    • Watson, L. E., Bates, P.L., Evans, T.M., Unwin, M.M. and Estes, J.R., 2002. Molecular phylogeny of Sub tribe Artemisiinae (Asteraceae), including Artemisia and its allied and segregate genera. Evolutionary Biology, 17 (2):1-12.
    • Wodehose, R.P., 1935. Pollen grains; their structure, identification and significance in science and medicine. McGraw-Hill Book Company. First edition.
    • Zare Chahouki, M.A., Jafari, M. and Azarnivand, H., 2008. Relationship between vegetation diversity and environmental factors in poshtkouh rangelands of Yazd Province. Pajouhesh-Va-Sazandegi, 21 (1): 192-199. (In Persian).
    • Zohary, M., 1973. Geobotanical Foundation of the Middle East. Gustav Fischer Verlag, Stuttgart, Germany.

     

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