Molecular and micromorphological study of Junipeus excelsa and J. polycarpos in the Chahar-Bagh of Golestan province, Iran

Document Type : Research Paper

Authors

1 MSc Graduted. Dept. Biology, Science College. Gonbad kavous university, Gonbad Kavous, I.R. Iran

2 Assist. Prof., Dept. Biology, Science College. Gonbad kavous university, Gonbad Kavous, I.R. Iran, Email Address: Abolfazl.daneshvar@gmail.com

3 Associate. Prof. Biology department, Science College. Gonbade kavous university, Gonbad Kavous, Iran

4 Assist. Prof. Dept. Biology, Science College. Gonbade kavous university, Gonbad Kavous, I.R. Iran

5 PhD Graduted. Science College. Qom University. Qom, I.R. Iran

10.22092/ijrfpbgr.2022.357415.1404

Abstract

Juniperus is one of the few conifers in Iran, which has six species and forms mostly sparse forests with scattered trees. Among them, Juniperus polycarpos and J.excela are more widespread than the other species. Due to the great similarity in their phenotypes, it is difficult to distinguish the two species from each other. In this study, pollinological, anatomical, and molecular indicators were evaluated to identify and differentiate these two Juniper species in the Chahar-Bagh region of Gorgan province, Iran. Using nrDNA ITS nuclear marker, molecular relationships between the two species were investigated. Phylogeny analyzes showed that two species are monophyletic and the populations were subdivided into unresolved subclades I and II. Pollen grains of the two species, J. polycarpos and J. excelsa, were examined using light (LM) and scanning electron (SEM) microscopes. The results showed that all pollen grains were monad, ulcurate, asymmetric, and small in size. In terms of shape, both species were spheroidal (prolate spheroidal) with granular exine ornamentations. The general shape of the pollens in the LM was circular with a smooth surface and no fovea, but the pollen grains examined under SEM were irregular and had fovea. The results of the anatomical studies of the stems also showed little difference, and it was not possible to distinguish and identify the two species. The results indicate that one species is probably distributed in the Chahar-Bagh region and these two taxons are synonymous.
 

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References

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