Micropropagation optimization of two endangered and endemic of Iran species of Ferulago (Ferulago subvelutina Rech.f. and Ferulago angulata (Schlecht) Boiss)

Document Type : Research Paper

Authors

1 PhD. Student, Department of Horticultural Science and Landscape Architecture, Ferdowsi University of Mashhad, Mashhad, I.R. Iran

2 Assist. Prof., Department of Ornamental Plants, Institute of Plant Science, Ferdowsi University of Mashhad, Mashhad, I.R. Iran.

3 Corresponding author, Assoc. Prof. Department of Horticultural Science and Landscape Architecture, Ferdowsi University of Mashhad, Mashhad, I.R.

Abstract

Ferulago (Ferulago ssp.) medicinal plant belongs to Apiaceae family and is endemic of Iran. Species of this genus are extremely endangered, considering the seed dormancy, excess harvesting and environmental stresses such as drought. By considering this plant as a valuable medicinal-rangeland plant, its production and reclamation is an essential task. This investigation was done to optimize micropropagation of two endemic species of Ferulago, included F. angulate and F. subvelutina. Maximum percentage of callus induction in F. angulata specie was reached as 100%, using rootlet explant (under treatments of 0 BAP + 1 NAA, 0 BAP + 1.5 NAA and 0 BAP + 2 NAA mg/l) and also true leaf explants (under treatments of 0.5 BAP + 0.5 NAA and 1 BAP + 2 NAA mg/l), and in F. subvelutina specie using true leaf explants (under treatments of 0.5 BAP + 0.5 NAA and 1 BAP + 2 NAA mg/l) respectively as 72.33 and 71.66%. The combined application of the cytokinin and auxin phytohormones has been shown to be effective in regenerating Ferulago species; so that the maximum regeneration was reached as 53.33 and 46.66% in F. angulata respectively under treatments of 0.5 BAP + 0.2 NAA mg/l and 0.5 BAP + 0.4 NAA mg/l and so on as 53.33 and 60 percent in F. subvelutina respectively under treatments of 1 BAP + 0.2 NAA mg/l and 1 BAP + 0.4 NAA mg/l. The best rooting treatment in both species was selected as the ½ MS cultural medium included 0.5 mg/l IBA. The adaptation stage was done in a mixture of soil, peat soil and sand with a weight ratio of 1: 1: 1, in which the total of 80% of regenerated plants from tissue culture, were successfully adapted to the outside environment.

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References

Abrazeh, M., 2003. Investigation of some characteristics of Ferulago angulata in Dena protected area. M.Sc. Thesis of Range Management, Department of Natural Resources, Tarbiat Modarres University, 72. (In Persian).
Ahmadloo, F., Goodarzi, G. and Salehi, A., 2017. Effect of growth regulators on callus induction and regeneration of Crataegus pseudohetrophylla from leaf segment. Journal of Medicinal Plants Biotechnology, 3(1): 14-22. (In Persian).
Alavi, M., Zare, N., Masumiasl, A., Sheikhzadeh Mosadegh, P. and Asghari Zakaria, R., 2020. Embryo culture to overcome seed dormancy and produce seedling in Ferulago angulata (Schlecht.) Boiss. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 28(1): 149-160. (In Persian).
Arikat, N.A., Jawad, F.M., Karam, N.S. and Shibli, R.A., 2004. Micropropagation and accumulation of essential oils in wild sage (Salvia fruticosa Mill.). Scientia Horticulturae, 100(1): 193-202.
Asgari, S. and Rafieian-Kopaei, M., 2015. Single or whole antioxidants in metal toxicities. Journal of Herbmed Pharmacology, 4(3): 69-70.
Bajaj, Y.P.S., Furmanowa, M. and Olszowska, O., 1988. Biotechnology of the micropropagation of medicinal and aromatic plants. In: Bajaj Y.P.S. (eds) Medicinal and Aromatic Plants I. Biotechnology in Agriculture and Forestry, vol 4. Springer, Berlin, Heidelberg. Pp. 60-103.
Baker, K.S., Steadman, K.J., Plummer, J.A., Merritt, D.J. and Dixon, K.W., 2005. The changing window of conditions that promotes germination of two fire ephemerals, Actinotus leucocephalus (Apiaceae) and Tersonia cyathiflora (Gyrostemonaceae). Annals of Botany, 96(7): 1225-1236.
Caboni, E., Meneghini, M. and Tonelli, M., 2010. Improved micropropagation of azarole (Crataegus azarolus L.). Propagation of Ornamental Plants, 10(1): 9-13.
Datta, M.M. and Jha, S., 2004. Embryo culture of Taxus wallichiana (Zucc.). Journal of Plant Biotechnology, 6(4): 213-219.
Dhivya, P., Sobiya, M., Selvamani, P. and Latha, S., 2014. An approach to Alzheimer’s disease treatment with cholinesterase inhibitory activity from various plant species. International Journal of Pharm Tech Research, 6(5): 1450-1467.
Ernst, D., 1989. Pimpinella anisum L. (Anise): Cell culture, somatic embryogenesis, and the production of anise oil. In: Bajaj Y.P.S. (eds) Medicinal and Aromatic Plants II. Biotechnology in Agriculture and Forestry, vol 7. Springer, Berlin, Heidelberg. Pp. 381-397.
Eyog-Matig, O., Aoudji, A.K.N. and Linsoussi, C., 2007. Garcinia kola Heckel seeds dormancy-breaking. Applied Ecology and Environmental Research, 5(1): 63-71.
Ghasempour, H.R., Shirinpour, E. and Heidari, H., 2007. The constituents of essential oils of Ferulago angulata (Schlecht.) Boiss at two different habitats, Nevakoh and Shahoo, Zagross Mountain, western Iran. Iranian Journal of Science and Technology (Sciences), 31(3): 309-312.
Golfakhrabadi, F., Khanavi, M., Ostad, S.N., Saeidnia, S., Vatandoost, H., Abai, M.R., Hafizi, M., Yousefbeyk, F., Rad, Y.R., Baghenegadian, A. and Ardekani, M.R.S., 2015. Biological activities and composition of Ferulago carduchorum essential oil. Journal of Arthropod-Borne Diseases, 9(1): 104.
Haghighat Hour, M., Asghari Zakaria, R. and Zare, N., 2012. Callus production and regeneration of medicinal plant Papaver pseudo-orientale under in vitro conditions. Iranian Journal of Plant Biology, 3(10): 11-22. (In Persian).
Hajimehdipoor, H., Shekarchi, M., Aghighi, A. and Hamzeloo-Moghadam, M., 2014. Evaluating the acetylcholinesterase inhibitory activity of Ferulago angulata and Ferulago subvelutina. Research Journal of Pharmacognosy, 1(2): 39-43.
Hosseini, N., Akbari, M., Ghafarzadegan, R., Changizi Ashtiyani, S. and Shahmohammadi, R., 2012. Total phenol, antioxidant and antibacterial activity of the essential oil and extracts of Ferulago angulata ssp. angulata. Medical Plant Journal, (43): 80-89.
Hritcu, L., Bagci, E., Aydin, E. and Mihasan, M., 2015. Antiamnesic and antioxidants effects of Ferulago angulata essential oil against scopolamine-induced memory impairment in laboratory rats. Neurochemical Research, 40(9): 1799-1809.
Jalili, A. and Jamzad, Z., 1999. A preliminary survey of endemic, rare and endangered plant Species in Iran. Research Institute of Forests and Rangelands Pressm Tehran, Iran, 759.
Jallilian, Z. and Ziarati, P., 2016. High potential of Ferulago angulate (Schlecht) Boiss. In adsorption of heavy metals. Biomedical and Pharmacology Journal, 9(1): 201-208.
Junairiah, M., Zuraidassanaaz, N.I., Izdihar, F.N. and Manuhara, Y.S.W., 2017. September. Callus induction of leaf explant Piper betle L. var. Nigra with combination of plant growth regulators indole-3-acetic acid (IAA), benzyl amino purin (BAP) and kinetin. In AIP Conference Proceedings (Vol. 1888, No. 1, p. 020028). AIP Publishing.
Khatibzadeh, R., Azizi, M., Arouiee, H. and Farsi, M., 2013. Effect of explants types and levels of plant growth substances on callogenesis in Levisticum officinale Koch. on a modified MS medium. Iranian Journal of Medicinal and Aromatic Plants Research, 29(2): 387-398. (In Persian).
Khawar, K.M., Sarhin, E.O., Sevimay, C.S., Cocu, S.A.T.I., Parmaksiz, I., Uranbey, S., Ipek, A., Kaya, M.E.H.M.E.T., Sancak, C. and Ozcan, S., 2005. Adventitious shoot regeneration and micropropagation of Plantago lanceolata L. Periodicum Biologorum, 107(1): 113-116.
Koohi, L., Zare, N., Asghari-Zakaria, R. and SheikhZadeh-Mosaddegh, P., 2014. The effect of plant growth regulators and different explants on the response of tissue culture and cell suspension cultures of german chamomile (Matricaria chamomilla L.). Journal of Crop Ecophysiology, 30(2): 203-214.
Leatherwood, W.R., 2005. Influence of Salt Stress on Germination, Root Elongation and Carbohydrate Content of Five Salt Tolerant Taxa, 114-241
Magyar-Tábori, K., Dobránszki, J., da Silva, J.A.T., Bulley, S.M. and Hudák, I., 2010. The role of cytokinins in shoot organogenesis in apple. Plant Cell, Tissue and Organ Culture (PCTOC), 101(3): 251-267.
Mahmoudi, R., Rafieirad, M. and Goudarzi, S., 2018 .Effect of hydro alcoholic extract of Ferulago angulata (Schlecht) Boiss on motor and memory disorders in animal model of parkinson disease. Qom University Medical Science Journal, 12(8): 36-47. (In Persian).
Mirzapour, S., Rafieirad, M. and Rouhi, L., 2015. Hydroalcoholic extract of Ferulago angulata improves memory and pain in brain hypoperfusion ischemia in rats. Jundishapur Journal of Natural Pharmaceutical Products, 10(1): 1-5.
Mortazavi, R., Dehdari, M. and Masoumiasl, A., 2016. Study of callus induction of medicinal Chavil plant (Ferulago angulata B.) using types of explants and growth regulators. Agricultural Biotechnology, 14 (2): 73-80. (In Persian).
Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3): 473-497.
Naghdi Badi, H., Salek Hashemi, S., Nasrollahnezhad Qomi, A., Navabpour, S. and Qaderi, A., 2017. Phytochemical evaluation and plant regeneration through somatic embryogenesis of Ferulago angulata subsp. Carduchorum (Boiss. and Hausskn), an endangered medicinal plant. Journal of Medicinal Plants, 16(63):92-107.
Narimani, R., Moghaddam, M. and Mojarab, S., 2016. Evaluation of the micropropagation of hairless catmint (Nepeta nuda L.), an endangered medicinal plant. Journal of Cell & Tissue, 7(4): 387-397. (In Persian).
Neibaur, I., Gallo, M. and Altpeter, F., 2008. The effect of auxin type and cytokinin concentration on callus induction and plant regeneration frequency from immature inflorescence segments of seashore paspalum (Paspalum vaginatum Swartz). In Vitro Cellular & Developmental Biology-Plant, 44(6): 480-486.
Okonkwo, T., 2015. Sustainable development and protection of endangered species fauna and flora in the wild in developing countries. International Journal of Development and Sustainability, 4(11): 1086-1115.
Omidi, M. and Farzin, N., 2009. Biotechnology approaches for improvement of medicinal plants. Food and Biotechnology Congress, Kermanshah Azad University, 1-14.
Pirbalouti, A.G., Sedaghat, L., Hamedi, B. and Tirgir, F., 2013. Chemical composition and antioxidant activity of essential oils of three endemic medicinal plants of Iran. Bangladesh Journal of Botany, 42(2): 327-332.
Rezazadeh, S., Yazdani, D. and Shahnazi, S., 2003. Chemical composition of essential oil of Ferulago angulata Boiss. Inflorescence From west of Iran. Journal of Medicinal Plants, 2(7): 49-52.
Sarabadani-tafreshi, R., Omidi, M., Bihamta, M.R. and Davazdahemami, S., 2008. Study of in vitro embryo culture and effect of explants, different hormonal levels on callus induction and shooting of Ferula gummosa. Journal of Medicinal Plants, 27: 71-81.
Sharifi, F., Rafieirad, M. and Sazegar, H., 2015. Effects of Ferulago angulata extract against oxidative stress induced by 6-hydroxydopamine in rats. Journal of Medicinal Plants, 1(53): 34-44.
Sharma, R.K., Wakhlu, A.K. and Boleria, M., 2004. Micropropagation of Anethum graveolens L through axillary shoot proliferation. Journal of Plant Biochemistry and Biotechnology, 13(2): 157-159.
Taran, M., Ghasempour, H.R. and Shirinpour, E., 2010. Antimicrobial activity of essential oils of Ferulago angulata subsp. carduchorum. Jundishapur Journal of Microbiology, 3(1): 10-14.
Zare, A.R., Solouki, M., Omidi, M., Irvani, N., Nezad, N.M. and Rezazadeh, S., 2010. Callus induction and plant regeneration in Ferula assafoetida L.(Asafetida), an endangered medicinal plant. Trakia Journal of Sciences, 8(1): 11-18.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 29, Issue 2 - Serial Number 58
February 2022
Pages 251-267

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