The effects of drought, salinity, and temperature stresses on the expression of menthone menthol reductase gene in Peppermint (Mentha piperita L.)

Document Type : Research Paper

Authors

1 Corresponding author, Assist. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran

2 M.Sc. Graduated, Department of Biology, Islamic Azad University, Tabriz Branch, Tabriz, I.R. Iran

Abstract

Peppermint (Mentha piperita) is one of the most important medicinal plants producing secondary metabolites. Menthol is an important component of peppermint essential oil monoterpenes, which is widely used in pharmaceutical and industrial applications. This monoterpene is produced by the Menthone Menthol Reductase (MMR) enzyme. Plant growth as well as the expression of this gene are affected by environmental stresses. In this study, peppermint rhizomes were first surface-sterilized and cultured in MS medium supplemented with sodium chloride (zero, 50, and 100 mM) and mannitol (zero, 50, 100, and 150 mM). Then they were placed in growth chambers at 23, 26, and 29°C. Three weeks after applying stresses, the expression of this gene in the leaves of the plants was measured by Real-Time PCR, and the obtained data were analyzed. According to the results, the expression of the MMR gene decreased at low concentrations of sodium chloride but increased by 97% at high concentration of 100 mM compared to the control. However, the expression of this gene has reached a minimum level of expression at high concentrations of mannitol as well as at high temperatures. Due to the importance of peppermint in the pharmaceutical industry, increasing the production of menthol through applying different treatments is important. Based on the obtained results, the treatment with mannitol (zero mM) and sodium chloride (50 mM) at 23°C increased the expression of the MMR gene by 93% compared to the control and it seems that the menthol production has reached its maximum level.
 

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References

Burbott, A. J. and Loomis, W. D., 1967. Effects of light and temperature on the monoterpenes of peppermint. Plant physiology, 42(1): 20-28.
Charles, D. J., Joly, R. J. and Simon, J. E., 1990. Effects of osmotic stress on the essential oil content and composition of peppermint. Phytochemistry, 29(9): 2837-2840.
Croteau, R. and Gershenzon, J., 1994. Genetic control of monoterpene biosynthesis in mints (Mentha: Lamiaceae). Genetic engineering of plant secondary metabolism, (New York: Plenum Press), pp. 193-229.
Croteau, R. B., Davis, E. M., Ringer, K. L. and Wildung, M. R., 2005. Menthol biosynthesis and molecular genetics. Naturwissenschaften, 92(12): 562.
Davazdahemami, S., 2003. Applications of Medicinal Plants (translation). Nosuh 113p. (In Persian), Isfahan, Iran
Davis, EM., Ringer, KL., McConkey, ME. and Croteau, R., 2005. Monoterpene metabolism: cloning, expression and characterization of menthone reductases from peppermint. Plant Physiology, 137:873–881.
Dow, A., Horning, E. and Cline, T. A., 1981. Salt tolerance studies on irrigated mint, Washington State University Agricultural Research Center, USA.
El-Keltawi, N. E. and Croteau, R., 1987. Salinity depression of growth and essential oil formation in spearmint and marjoram and its reversal by foliar applied cytokinin. Phytochemistry, 26(5): 1333-1334.
Elyasi, R., Majdi, M., Bahramnejad, B. and Mirzaghaderi, Gh., 2016. Expression analysis of genes involved in terpenes biosynthesis in black cumin (Nigella sativa) plants treated with methyl jasmonate. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(1): 54-65. (In Persian)
Farooqi, A., Samgwan, N. and Sangwan, R., 1999. Effect of different photoperiodic regimes on growth, flowering and essential oil in Mentha species. Plant Growth Regulation, 29(3): 181-187.
Faure, O., Diemer, F., Moja, S. and Jullien, F., 1998. Mannitol and thidiazuron improve in vitro shoot regeneration from spearmint and peppermint leaf disks. Plant cell, Tissue and Organ Culture, 52(3): 209-212.
Ghobadi, S., Maroufi, A. and Majd, M., 2017. Differential expression of the key genes involved in the biosynthesis of monoterpenes in different tissues and in response to abiotic elicitors in Summer savory (Satureja hortensis). Journal of Cell and Tissue, 7(3): 275-292.
Gobert, V., Moja, S., Colson, M. and Taberlet, P., 2002. Hybridization in the section Mentha (Lamiaceae) inferred from AFLP markers. American Journal of Botany, 89(12): 2017-23.
Mucciarelli, M., Camusso, W., Bertea, C. M., Bossi, S. and Maffei, M., 2001. Effect of (+)-pulegone and other oil components of Mentha×piperita on cucumber respiration. Phytochemistry, 57(1): 91-98.
Naderi, S., Fakheri, B. and Khaje, H., 2015. The effect of chitosan on menthol dehydrogenase gene expression and menthol content in peppermint (Mentha piperita L.) by Real time PCR. Journal of Medicinal Plants Biotechnology. 1(1): 23-32.
Pfaffl, M.W., Horgan, G.W. and Dempfle, L., 2002. Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 30: 1-10.
Seyed Rahmani1, R., Naghavi, M.R., Mohammadi, V. and Ranjbar, M., 2014. Relationship between expression of main MEP pathway genes and monoterpenes contents in Artemisia annua. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 22(2): 192-200. (In Persian).
Shamsi-Fard, M.H., Mirzaghaderi, G. and Majdi, M. 2014. Transcript expression analysis of geranyl diphosphate synthase gene  in different tissues of black cumin (Nigella sativa L.). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 22(2): 143-155. (In Persian).
Soleymani, F., Taheri, H. and Shafeinia, A. 2017. Relative expression of genes of menthol biosynthesis pathway in peppermint (Mentha piperita L.) after chitosan, gibberellic acid and methyl jasmonate treatments. Russian Journal of Plant Physiology, 64(1): 59-66.
Wang, Q., Reddy, V. A., Panicker, D., Mao, H. Z., Kumar, N., Rajan, C., Venkatesh, P. N., Chua, N. H. and Sarojam, R., 2016. Metabolic engineering of terpene biosynthesis in plants using a trichome‐specific transcription factor Ms YABBY 5 from spearmint (Mentha spicata). Plant biotechnology Journal, 14(7): 1619-1632.
Zhang, L. and Barritt, G. J., 2004. Evidence that TRPM8 is an androgen-dependent Ca2+ channel required for the survival of prostate cancer cells. Cancer research, 64(22): 8365-8373.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 29, Issue 2 - Serial Number 58
February 2022
Pages 196-206

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