Evaluation of morphological traits and anti-microbial activity of local ecotypes of Mentha longifolia in Lorestan, Iran

Document Type : Research Paper

Author

Assist. Prof., Department of Agriculture, Payame Noor University, Tehran, Iran.

Abstract

In order to evaluation the diversity of morphological traits and determine antibacterial activity against three bacterial strains including Klebsiella pneumonia, Listeria monocytogenes, and Streptococcus agalactia, three ecotypes of Mentha longifolia were collected from three habitats in Khorramabad, Aleshtar, and Delfan in Lorestan province, Iran. The measured traits were plant height, leaf length and width, number of leaves, branches, and inflorescences, length of inflorescences, and percentage of essential oil. Data were analyzed using one-way ANOVA with five replications. The result showed that there was a significant difference among the ecotypes in terms of leaf length and width, plant height, and essential oil percentage. Khorramabad ecotype had the highest plant height, inflorescence length, and essential oil percentage. A positive and significant correlation was observed between both plant height and leaf width with essential oil percentage. According to stepwise regression and path analysis results, it was concluded that plant height could be considered as the most important index for the selection of parental lines in the breeding programs. Based on the Minimum Inhibitory (MIC) and Minimum Bactericidal Concentration (MBC) indices, it was concluded that the minimum inhibitory and bactericidal concentrations of M. longifolia essential oil ranged from 0.03 to 0.2 mg/mL.The antimicrobial activity showed that L. monocytogenes and S.agalactiae were inhibited by Delfan ecotype essential oil and K. pneumonia was inhibited by the essential oil from the Aleshtar ecotype. In contrast, the three studied bacteria had the lowst susceptibility to the essential oil from Khorramabad ecotype.

Keywords

Main Subjects

References

  • Abbaszadeh, B., Rezaee, M. and Layegh Haghighi, M., 2012. Investigation morphological characteristics and essential oil component of 2 ecotypes of watermint (Mentha aquatica). Journal of Medicinal Plants, 1 (41): 248-257. (In Persian).
  • Abou El-Nasr, T.H.S., Ibrahim, M.M., Aboud, K.A. and El-Enany, M.A.M., 2013. Assessment of genetic variability for three corianders (Coriandrum sativum) cultivars grown in Egypt, using morphological characters, essential oil composition and ISSR markers. World Applied Science Journal, 25 (6): 839-849.
  • Adams, R.P., 2001. Identification of essential oil components by gas chromatography and Mass Spectrometry. Allured, USA. 750p.
  • Anwar, F., Alkharfy, K.M., Rehman, N., Adam, E.H.K. and Gilani, A.H., 2017. Chemo-geographical variations in the composition of volatiles and the biological attributes of Mentha longifolia essential oils from Saudi Arabia. International Journal of Pharmacology, 13 (5): 408-424.
  • Atashgahi, Z., Ejtehadi, H. and Zare, H., 2009. Study of florists, life from and chorology of plants in the east of Dodangeh forests, Mazandaran province, Iran. Iranian Journal of Biology, 22 (2): 193-203. (In Persian)
  • Azarkish, P., Moghaddam, M., Vaezi, J., Ghasemi Pirbalouti, A. and Davarinejad, Gh., 2016. Genetic diversity of ecotypes of horse mint (Mentha longifolia) in the southwest of Iran using morphological traits. Seed and Plant Improvement Journal, 32 (3): 311-329. (In Persian)
  • Bajalan, I. and Pirbalouti, A.G., 2014. Variation in antibacterial activity and chemical compositions of essential oil from different populations of Myrtle. Industrial Crops and Products, 61: 303-307.
  • Basheer, N., Modawi, A., Elamin, H.B. and Ibrahim, H.M., 2015. A potential new isolate for streptokinase production. International Journal of Current Microbiology and Applied Sciences, 4: 380-7.
  • Behera, T.K., Gaikward, A.B., Singh, A.K. and Staub, J.E., 2008. The relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia ). Journal of the Science of Food and Agriculture, 88: 733-737.
  • Giamarellou, H. and Poulakou, G., 2009. Multidrug-resistant gram-negative infections. Drug, 69 (14): 1879-1901.
  • Glisic, S.B., Milojevic, S.Z., Dimitrijevic, S.I., Orlovic, A.M. and Skala, D.U., 2007. Antimicrobial activity of the essential oil and different fractions of Juniperus communis and a comparison with some commercial antibiotics. Journal of the Serbian Chemical Society, 72: 311-320.
  • Hadian, J., Karimi, E., Shouryabi, M., Nadjafi, F. and Kanani, M.R., 2016. Evaluation of morphological variation and path coefficient analysis of oil content of Thymus daenensis Celak populations. Plant Production Technology, 16 (1): 41-56. (In Persian)
  • Hasanpour Reihani, K., Ghasemi, R. and Sofalian, A., 2016. Study of morphological variation of Mentha longifolia The 5th National Conference on Agricultural and Sustainable Natural Resources, Tehran, Iran. (In Persian)
  • Hof, H., Nichterlein, T., Lampidis, R. and Wecke, J., 1998. Listeria dispose of many facettes. Biotest Bulletin, 6: 21-3.
  • Hosseini, S.A. and Dori, M.A., 2005. Preliminary investigation on the establishment and performance of flower of Hypericum perforatum collected from Diarrhoea and Garmabad in the Golestan province. Iranian Journal of Medicinal and Aromatic Plants, 20 (4): 397-406. (In Persian)
  • Hosseini, Z., 2016. Evaluation of morphological and phytochemical characteristics of Mentha longifolia during flowering in Fars and Khorasan Razavi province. M.Sc. Thesis. University of Torbat Heydarieh, Iran. (In Persian)
  • Jorgensen, H. 1993. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard. Wayne, PA:National Committee for Clinical Laboratory Standards Antimicrobial Susceptibility Testing. NCCLS M7-A3.
  • Kalemba, D. and Kunicka, A., 2003. Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry; 10 (10): 813-839.
  • Khanuja, S.P.S., Shasany, A.K., Srivastava, A. and Kumar, S., 2000. Assessment of genetic relationships in Mentha Euphytica, 111: 121-125.
  • Koba, K., Sanda, K., Raynaud, C.D., Nenonene, Y.A., Millet, J. and Chaumont, J.P., 2004. Antimicrobial activities of essential oils from African cymbopogon against microorganisms pathogenic in pets. Annales de Médecine Vétérinaire, 148, 202-206
  • Masomi, J., Yadegari, D. and Mozoni, Sh., 2005. More appropriate antimicrobial agents for antibiogram. Iranian Journal of Infectious Diseases and Tropical Medicine, 10 (29): 53-58.
  • Masoumipour, F., Hassanshahian, M. and Jafarinasab, T., 2018. Antimicrobial activity of combined extracts of Trachyspermum, Thymus and Pistachio against some pathogenic bacteria. Journal of Kerman University of Medical Science, 25(2): 153-63.
  • Mian, G.F., Godoy, D.T., Leal, C.A.G., Yuhara, T.Y., Costa, G.M. and Figueiredo, H.C.P., 2009. Aspects of the natural history and virulence of S.agalactiae infection in Nile tilapia. Veterinary Microbiology Journal, 136: 180-183.
  • Mohsenipour, Z. and Hassanshahian, M., 2016. Antibacterial activity of euphorbia hebecarpa alcoholic extracts against six human pathogenic bacteria in planktonic and biofilm forms. Jundishapur Journal of Microbiology, 9 (6): 1-7.
  • Moshtaghi, H. and Boniadian, M., 2008. The effect of oil of Mentha spicata on Listeria in food model. Iranian Journal of Medicinal and Aromatic Plants, 24 (3): 326-332.
  • Mousavi,M. and Hemmati, Kh., 2014. Effect of height on essential oil of Mentha longifolia L. in Chaharmahal va Bakhtiari province. Natioal Conference of Medicinal Plant, Islamic Azad University, Ayatollah Amoli Branch. Tehran. (In Persian).
  • Pourali Sheshblouki, G.H., Mardaneh, J. and Hosseinzadeh, Z., 2016. Klebsiella pneumonia infections in hospitalized patients: characterization of antibiotic cross-resistance and detection of cefepime susceptible-dose dependent strains. Journal of Fasa University of Medical Sciences, 6 (1): 52-59. (In Persian)
  • Rahimi, M.H., Aharizad, S. and Mohebalipour, N., Evaluation of genetic diversity in populations of Lemon balm (Melissa officinalis) in terms of agronomic traits, essential oil and central concentration. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 25 (2): 271-288. (In Persian)
  • Saadatjou, B., Mohammadkhani, A., Saeidi, K. and Shirmaridi, H.A., 2015. The evaluation of morphological variation and essential oil content of Salvia species ecotypes in south-west of Iran. Applied Crop Breeding, 3 (1): 125-135. (In Persian)
  • Sadeghian, I., Hassanshahian, M., Sadeghian, S. and Jamali, S., 2012. Antimicrobial effects of Quercus Brantii fruits on bacterial pathogens. Jundishapur Journal of Microbiology, 5 (3): 465-9.
  • Shahbazi, Y., 2015. Chemical composition and in vitro antibacterial activity of Mentha spicata essential oil against common food-borne pathogenic bacteria. Journal of Pathogens: 1-5.
  • Shan, B., Cai, Y.Z., Brooks, J.D. and Corke, H., 2007. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. International Journal of Food Microbiology, 117(1): 112-119.
  • Singh, R., Shushni, M.A.M. and Belkheir, A., 2015. Antibacterial and antioxidant activities of Mentha piperita. Arabian Journal of Chemistry, 8: 322-328.
  • Singh, S.P., Sharma, and Tewari, R.K., 1998. Genetic improvement of Mentha arvensis based on essential oil yield and quality traits. Journal of Herbs, Species and Medicinal Plants, 6 (2): 79-86.
  • Strycharz, S. and Shetty, K., 2002. Peroxidase activity and phenolic content in elite clonal lines of Mentha pulegium in response to polymeric dye R-478 and Agrobacterium rhizogenes. Process Biochemistry, 37 (8): 805- 12.
  • Tserennadmid, R., Tako, M., Galgoczy, L., Papp, T., Vagvolgyi, C., Gero, L. and Krisch, J., 2010. Antibacterial effect of essential oils and interaction with food components. Central European Journal of Biology, 5(5): 641-648.
  • Tsuchiya, H., Sato, M., Miyazaki, S., Fujiwara, S., Tanigaki, S., Ohyama, M., Tanaka, T. and Linuma, M. 1996. Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology, 50 (1): 27-34.
  • Vining,J., Zhang, Q., Tucker, A.O., Smith, C. and Davis, T.M., 2005. Mentha longifolia a model species for mint genetic research. Horticultural Science, 40 (5): 1225-1229.
  • Zeinali, H., Vali, E., Safaei, L. and Enteshari, Sh., 2012. Study of reproductive trait variation in Mentha Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 20 (2): 327-339. (In Persian)
  • Zinodini, A., Farshadfsr, M., Safari, H., Moradi, F. and Shirvani, H., 2013. Study of genetic relationships of some Mint species using ISSR markers. Crop Biotechnology, 3 (5): 11-21. (In Persian)
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 29, Issue 1 - Serial Number 57
September 2021
Pages 96-107

Files

Share

How to cite

Statistics