Stimulatory effects of Thidiazuron, Putrescine, and explant type on indirect regeneration of Calendula officinalis L.

Document Type : Research Paper

Authors

1 M.Sc. graduated, Department of Horticulture Science, Khuzestan Agricultural Sciences and Natural Resources University, Mola-Sani, Khuzestan, I.R.Iran.

2 Corresponding author, Assoc. Prof., Department of Horticulture Science, Khuzestan Agricultural Sciences and Natural Resources University, Mola-Sani, Khuzestan, II.R. Iran, Email: mrsalehisalmi@gmail.com

3 Prof. Department of Horticulture Science, Khuzestan Agricultural Sciences and Natural Resources University, Mola-Sani, Khuzestan, I.R.Iran.

Abstract

Pot marigold (Calendula officinalis L.) is an important ornamental-medicinal plant in Iran, that is widely used alone or in combination with other medicinal to produce low-risk drugs for human. In order to mass-production of this plant by tissue culture, four separate factorial experiments were conducted using completely randomized design with three replications (10 samples) at four stages of callus induction, indirect organogenesis, rooting, and acclimatization The results showed that the highest callus weight was observed in the interaction of leaf explant and medium supplemented with 2,4-D (2.0 mg/l). The tallest and highest branching number was related to the callus obtained from leaf explant in medium containing thidiazuron (1.0 mg/l TDZ) and putrescine (100 mg/l PUT), in indirect organogenesis. Tissue culture cuttings had the best rooting in 2.0 mg/l NAA medium. The obtained seedlings planted in pots containing perlite showed better acclimatization. By optimizing callus production from Calendula officinalis explants and propagation by tissue culture method can be an important step towards producing plants with the same quality and free of pathogens.

Keywords

Main Subjects

References

-  Adela, P., Constantin, B., Sanda, A. and Carmen, S., 2003. HPLC analysis of carotenoids in four varieties of Calendula officinalis L. flowers. Acta Biological, 47: 37-40.
-  Ali, R. M., Abbas, H. M. and Kamal, R. K., 2009. The effect of treatment with polyamines on dry matter and some metabolites in salinity stressed chamomile and sweet majoram seedling. Plant and soil environment, 11: 477-483.
-  Arora, D., Rani, A. and Sharma, A., 2013. A review on phytochemistry and ethnopharma-cological aspects of genus Calendula. Pharmacognosy Reviews, 7: 179-187.
-  Bazrafkan, M., Daneshvar, M. H. and Salehi Salmi, M. R. 2019. Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of sweet pea (Lathyrus odoratus L.). Iranian Journal of Rangelands and Forests Pant Breeding and Genetics Research, 27: 98-107.  (In Persian).
-  Bhatia, R., Singh, K. P., Jhang, T. and Sharma, T. R., 2009. Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers. Scientia Horticulturae , 119: 208-211.
-  Gazim, Z. C., Rezende, C. M., Fraga, S. R., Svidzinski, T. I. E. and Cortez, D. A. G., 2008. Antifungal activity of the essential oil from Calendula officinalis L. (Asteraceae) growing in Brazil. Brazilian Journal Microbiological, 39: 61-63.
-  Huetteman, A. and Preece, J., 1993. Thidiazuron a potent cytokinin for wood plant tissue culture. Plant cell, tissue and organ culture, 37:105-119.
-  Jafari, S., Daneshvar, M. H., Salehi Salmi, M. R. and Lotfi, A. 2017. Influence of putrescine and thidiazuron on in vitro organogenesis in Salvia officinalis L. Iranian Journal of Rangelands and Forests Pant Breeding and Genetics Research, 25: 201-211. (In Persian).
-  Jegadeeswari, V., Indurani, C. and Kalaiselvi, T., 2007. Calendula: The poor manˊs Saffron. Science Technology. Entrepreneur. Tamil Nadu Agricultural University, Coimbatore. Pharmaceutical Bulletin, 49: 863-870.
-  Leal, F., Matos, M., Coelho, A. C. and Pinto-Carnide, O., 2012. In vitro Multiplication of Aromatic and Medicinal plant and Fungicide activity. Journal of Agricultural and Biological Science, 6: 119- 138.
-  Legha, M. R., Prasad, V., Singh, S. K., Kaur, C., Arora, A. and Kumar, S., 2012. Induction of carotenoid pigments in callus cultures of Calendula officinalis L. In response to nitrogen and sucrose levels. In Vitro Cellular & Developmental Biology-Plant, 48: 99-106.
-  Marks, T. R. and Simpson, S. E., 1994. Factors affecting shoot development in apically dominant Acer cultivars in vitro. Journal of Horticultural Science, 69: 543-551.
-  Misra, P., Purshottam, D. K., Siddiqui, S., Jain, M. B. and Toppo, D. B. A., 2010. A comparative study of in vitro regeneration of shoots in different cultivars of Gerbera jamesonii H. Bollus ex Hookf. Propagation of Ornamental Plants, 10: 156-162.
-  Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassay with tissue culture. Physiologia Plantarum, 15: 473-476.
-  Paradiso, R. and De Pascale, S. 2008. Effects of coco fiber addition to perlite on growth and yield of cut gerbera. Acta Horticulture, 779: 529-534.
-  Razdan, M. K., 2003. Introduction to plant tissue culture. Oxford of IB publish. Dehli. India, 23: 256-261.
-  Shailaja, V. P., 2002. Studies on in vitro propagation of Gerbera jamesonii “Bolus”. Dharwad (India): M. Sc. Thesis of the University of Agricultural Sciences. Dharwad. India.
-  Son, N. V. 2007. Response of gerbera (Gerbera jamesonii “Bolusˮ) varieties to micropropogation. Dharwad (India): M. Sc. Thesis of the University of Agricultural Sciences. Dharwad. India.
-  Taha, R. M., Hasbullah. N. A., Abdul Aziz, A. H. and Awal, A., 2010. Establishment of in vitro plantlets and acclimatization of Gerbera jamesonii Bolus ex. Hook f. Acta Horticulture, 865: 401-404.
-  Victoria, C. P., Lage, C. L. S. and Sato, A., 2012. Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis. Revista Ciencia Agronomica, 43: 539-545.
-  Zebarjadi, A. R., Motamedi, M. J., Taravat, E. and Ismaili, A. 2013. Micropropagation of medicinal purple coneflower (Echinacea purpurea L.) using cotyledon and hypocotyl segments. Journal of Plant Research, 26: 311-319.
Jimenez-Medina, E., Garcia-Lora, A., Paco, L., Algarra, I., Collado, A. and Garridoet, F., 2006, A new extract of the plant Calendula officinalis produces a dual in vitro effect: Cytotoxic anti-tumor activity and lymphocyte activation. BMC Cancer, 119: 1-14

Files

Share

How to cite

Statistics