Embryo culture to overcome seed dormancy and produce seedling in Ferulago angulata (Schlecht.) Boiss.

Document Type : Research Paper

Authors

1 Ph.D. Graduated, Department of Agronomy and Plant Breeding, Faculty of Agriculture Natural Resources, University of Mohaghegh Ardabili, Ardabil, I.R. Iran.

2 Assoc. Prof., Department of Agronomy and Plant Breeding, Faculty of Agriculture Natural Resources, University of Mohaghegh Ardabili, Ardabil, I.R. Iran.

3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Yasouj, Yasouj, I.R. Iran.

4 Assist. Prof., Department of Agronomy and Plant Breeding, Faculty of Agriculture Natural Resources, University of Mohaghegh Ardabili, Ardabil, I.R. Iran.

5 Prof., Department of Agronomy and Plant Breeding, Faculty of Agriculture Natural Resources, University of Mohaghegh Ardabili, Ardabil, I.R. Iran

Abstract

 Ferulago angulata as a medicinal plant belongs to the Apiaceae family, and propagated by seed. But, seed dormancy is a main limiting factor in its propagation. To evaluate the efficiency of in vitro culture as well as the combination of culture medium and explant type on breaking seeds dormancy of Ferulago angulata, a factorial experiment was conducted based on a completely randomized design with five replications. The treatments consisted of four ecotypes (Abenahr, Gauonhe, Vezg, Sisakht) from Kohgiluyeh and Boyer-Ahmad province, Iran and three concentrations of Murashige and Skoog basal medium (1/4, 1/2 and 1MS) and explant type (seed or embryo). Collected seeds were surface sterilized and then were planted as intact seeds, and isolated embryos in the above mentioned media. In this experiment, isolated embryos germinated after 3 to 6 days, depending on the ecotype. However, no germination was observed in the intact seeds in the used media. The results of embryo culture showed that culture medium and ecotype significantly affected all measured seedling traits. Mean comparison indicated that there were significant differences between different media and 1/4MS medium was superior to the other two culture media in terms of embryo germination percentage and seedling growth. There also were significant differences between different ecotypes, and Gauonhe ecotype showed higher embryo germination percentage and seedling growth than other ecotypes. So, in vitro culture of Ferulago angulata seed embryos on 1/4MS medium provides an effective method to overcome seed dormancy. Seedlings resulting from germination and embryo growth were successfully transferred to pots and placed in natural environment. According to the results of this study, germination inhibitors in F. angulata are located in the tissues surrounding the embryo and the embryo would not be able to germinate until the inhibitory factors are removed. Therefore, isolation and in vitro culture of the embryo provides a suitable approach to overcome seed dormancy in this plant, especially for laboratory studies.

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References

Amooaghaie, R., 2005. The effect of soaking, temperature and duration of pre-chilling on seed dormancy breaking of Ferula ovina. Iranian Journal ofBiology, 18(4): 350-359. (In Persian).
-Bagheri, A. and Safari, M., 2008. Basics of Plant Tissue Culture. Publication of Ferdowsi University of Mashhad, 424p. Mashhad, Iran. (In Persian).
-Baskin, J.M. and Baskin, C.C., 2004. Classification system for seed dormancy. Seed Science Research, 1: 14–16.
-Bridgen, M.P., 1994. A review of plant embryo culture. Hortscience, 29(11): 1243-1246.
-Craverol, V. and Cointry, E. 2007. Shortening the seed to seed cycle in artichoke breeding by embryo culture. Plant Breeding, 126: 222-224.
-Copeland, L.O. and McDonald, M., 2001. Principles of Seed Science and Technology. Kluwer Academic Publisher, Norwell, 488p.
-Ehsanpour, A.A. and Zahedi, S, 2006. Induction of somatic embryogenesis from cotyledon of oak (Quercus castaneifolia). Journal of Science (University of Tehran), 32(1): 47-50.
-Finch-Savage, W.E. and Leubner-Metzger, G., 2006. Seed dormancy and the control of germination. New Phytologist, 171: 501–523.
-Fu, X.X., Liu, H.N., Zhou, X.D. and Shang, X.L., 2013. Seed dormancy mechanism and dormancy breaking techniques for Cornus kousa var. Chinensis. Seed Science and Technology, 41: 458-463.
-Ghahreman, A., 1986. Flora of Iran. Research Institute of Forest and Rangelands publication, Tehran, Iran, No, 17 (2048). (In Persian).
-Ghasemian, K.h., Nazeri, S., Chehregani-Rad, A. and Mirzaie-Asl, A. 2012. The stages of somatic embryogenesis derived from zygotic embryo of Dorema ammoniacum D. Journal of Cell and Tissue (JCT), 3(1): 21-27. (In Persian).
-Gupta, V., 2003. Seed germination and dormancy breaking techniques for indigenous medicinal and aromatic plants. Journal of Medicinal and Aromatic Plants Science, 25: 402-407.
-Jafari, M. and Daneshvar, M.H., 2016. Indirect organogenesis of Tagetes erecta L. via hypocotyl explant. Scientific Journal of Ornamental Flowers and Ornamental Plants, 1(2): 34-43. (In Persian).
-Jafari-Mofidabadi, A. and Amani, M., 2004. Seed dormancy breakage of Fagus orientalis Lipsky using embryo culture. Iranian Journal ofRangelands and Forests Plant Breeding and Genetic Research, 12(3): 257-274. (In Persian).
-Koohi, L., Zare, N., Asghari-Zakaria, R. and SheikhZadeh-Mosaddegh, P., 2014. The effect of plant growth regulators and different explants on the response of tissue culture and cell suspension cultures of german chamomile (Matricaria chamomilla L.). Journal of Crop Ecophysiology, 8: 203-214. (In Persian).
-Modares, M., Abrishamchi, P., Ejtehadi, H. and Ramezani, A. 2007. Propagation of Salvia leriifolia Benth. by embryo culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 15(2): 129-41. (In Persian).
-Montazeri, F., Omidi, M. and Imani, N., 2009. Comparing various methods of seed and embryo culture and charcoal effect in optimizing in vitro culture of Galbanum (Ferula gummosa). Iranian Journal of Medicinal and Aromatic Plants, 26(4): 583-595. (In Persian).
-Mortazavi, R., Dehdari, A., Masumiasl, A. and Fayyaz, P., 2013. Seed dormancy of Ferulago angulata (Schlecht). Boiss by using embryo culture in various media cultures. 8th National Biotechnology Congress of Islamic Rpublic of Iran and 4th National Conference on Biosecurity. University of Tehran, Tehran, Iran. 6-8 July. 2013: 1-4. (In Persian).
-Movahedi-dehnavi, M., 2011. Effect of seed drop breeding treatments on germination and plant growth of Ferulago angulata, Celery, Iranian Cumin, Halibut, Saree and Cumin. Final Report of the Research and Technology Project, Yasouj University, Iran. (In Persian).
-Mozafriyan, V., 2006. Glossary of Iranian Plants Names (1). Tehran, Farhang Mooser Publishing, Volume I, 594p. (In Persian).
-Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiolagia Plantarum, 15: 473-497.
-Phillips, N., Drost, D. and Varga, V., 2003. Chemical treatments enhanced seed germination in Perideridia gairdneri. International Society for Horticultural Science, 618: 477-482.
-Sanatombi, K. and Sharma, G.J., 2008. In vitro plant regeneration in six cultivars of Capsicum spp. using different explants. Biologica Plantrum, 52: 141-145.
-Sarabadani-tafreshi, R., Omidi, M., Bihamta, M.R. and Davazdahemami, S., 2008. Study of in vitro embryo culture and Effect of explants, different hormonal levels on callus induction and shooting of Ferula gummosa. Journal of Medicinal Plants, 27: 71-81. (In Persian).
-Sharifi, H., Khajeh-hosseini, M. and Rashed-mohassel, M.H., 2015. Study of seed dormancy in seven medicinal species from Apiaceae. Iranian Journal of Seed Research, 2(1): 25-36. (In Persian).
-Taghinezad, Z., Dehdari, M., Mirshkari, A. and Zaynali, H., 2016. Effect of gibberllic acid, temperature and embryo culture on seed germination of four native species of Barberry (Berberis spp.). Iranian Journal of Seed Research, 3(1): 27-37. (In Persian).
-Taran, M., Ghasempour, H.R. and Shirinpour, E., 2010. Antimicrobial activity of essential oils of Ferulago angulata. Jundishapur Journal of Microbiology, 3(1): 10-14.
-Zare, N., Valizadeh, M., Tohidfar, M., Mohammadi, S.A., Malboobi, M.A. and Habashi, A.A., 2009. Selection of regenerative genotypes from Iranian alfalfa cultivars. Journal of food, agriculture and environment, 7: 567-572.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 28, Issue 1 - Serial Number 55
September 2020
Pages 149-160

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