Assessment of genetic diversity of Anthemis haussknechtti, A. pseudocutula and  A. triumfetti populations using electrophoretic pattern of total proteins

Zekri, Maryam Alsadat; Salehi-shanjani, Parvin; Javadi, Hamideh; Alizadeh, Ali

doi:10.22092/ijrfpbgr.2015.12235

Assessment of genetic diversity of Anthemis haussknechtti, A. pseudocutula and A. triumfetti populations using electrophoretic pattern of total proteins

Document Type : Research Paper

Authors

10.22092/ijrfpbgr.2015.12235

Abstract

Chamomile, a herbaceous and perennial plant from Asteracaea and Anthemis is the second largest genus in the family. Knowledge of genetic diversity and differentiation related genes, genotypes and knowledge of how to operate, is essential for breeding programs and may play an important role in genetics, biochemistry and plant breeding .Therefore, genetic diversity of 90 genotype of Anthemis (one population of A. pseudocotula, two populations of A. haussknechtti and six populations of A. triumfetti) were studied by electrophoretic pattern of total proteins and investigated to determine extent of genetic diversity. On the basis of SDS-PAGE, 24 reproducible bands were observed. A. haussknechtti and A. triumfetti species had highest number of bands (23) and A. pseudocutula had minimum number of bands (17). Percentage of polymorphism was 49/07% and specific band was observed. Longest distance was observed between A. haussknechtti (Ivan) and A. triumfetti (Ramian). Analysis of molecular variance showed that genetic differentiation between and within the populations were 57% and 43% respectively. Correlation between the protein data, latitude and longitude showed significant differences (R²=/03049 and P=0/010).

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