Optimization of regeneration in Mountain Ziziphora (Ziziphora clinopodioides Lam)

Document Type : Research Paper

Authors

1 - Msc. Plant Biotechnology. Complex Higher education of Shirvan. North Khorasan, I.R. Iran.

2 Assistant professor. Plant Biotechnology. Complex Higher education of Shirvan. North Khorasan, I.R. Iran.

3 Assistant professor. Plant Biotechnology. Research Center for Plant Sciences. Ferdowsi University of Mashhad. I.R. Iran.

4 Associate Professor. Agronomy. Complex Higher education of Shirvan. North Khorasan, I.R. Iran

Abstract

DOR: 98.1000/1735-0891.1398.27.144.53.1.1576.41
Medicinal and aromatic medicinal plant, Ziziphora clinopodioides L., belonging to the genus Ziziphora and Lamiaceae family. The aim of this study was to optimize asexual regeneration in two genotypes of this species as code 5 and 6. For this purpose, the first experiment was conducted for contamination control using sodium hypochlorite 1% and alcohol 96%. In the next step, the control of produced phenolic compounds was investigated by combining three polyvinylpyrrolidone, active charcoal and ascorbic acid. Multiple shoots induction were performed using lateral bud explant in a MS medium containing the BAP at five levels in combination with NAA (0.01 mg/L). Rooting of branches was performed in MS medium containing NAA at two levels (0. 1 and 0.3 mg/L). Result showed 1% sodium hypochlorite treatment coupled with 96% alcohol eliminated contamination up to 74%. The treatment of combination of polyvinylpyrrolidine, ascorbic acid and activated charcoal with average value of 8.25% had the lowest browning in both Genotype of 6 and 5. The highest branching values of (88% and 100%) were obtained in the treatment of 2 and 2.5 mg/L BAP combined with 0.01 mg/L NAA in genotype 5 and 6, respectively. The highest value of rooting (33.3%) was observed in genotype 6 using 0.3 mg/l NAA. The results of this research could be  used in the micropropagation and proliferation of this plant.

Keywords

Main Subjects


-  Asadi, A.A., Vedadi, C., Rahimi, M. and Naserian Khiabani, B., 2009. Effect of plant growth hormones on root and shoot regeneration in Rose (Morrasia) under in vitro conditions, Bioscience Research, 6(1): 40-45.
-  Bakhshi Kaniki, G.R., Sefidkan, F. and Dehghan, Z., 2010. Investigation of the effect of some habitat conditions on the quality and quantity of essential oils of Ziziphora clinopodioides, Herbal Medicines, 1: 11-20
-  Baser, K.H.C., Sezik, E., Tumen, G., 1991. Composition of the Essential Oil of Ziziphora clinopodioides Lam. Journal of Essential Oil Research, 3:237-239.
-  Beikmohammadi, M. 2011. The evaluation of medicinal properties of Ziziphora clinopodioides, Journal World Applied Sciences, 12(9):1635-1638.
-  Cowan, M. M. 1999. Plant products as antimicrobial agents. Clinical Microbiology, 12(4):564-582.
-  Elmore, H., Samples, B., Sharma, S., and Harrison, M. 1990. Influence of cultural and physiochemical factors on ascorbate stability in plant tissue culture media. Plant Cell, Tissue and Organ Culture, 20: 131-135.
-  Eisenman, S.W., Zaurov, D. F., and Struwe, L. 2012. Medicinal plant of Central Asia: Uzbekistan and Kyrgyzstan, Springer New York Heidelberg Dordrecht London, 351p
Hirata, T., Murakami, S., Ogihara, K. and Suga, T. 1990. Volatile monoterpenoid constituent of the plantlet of Mentha spicata produced by shoot tip culture. Phytochemistry, 29(2): 493-495.
-  Hosseini, B. and Alizadeh, M. 2013. Effect of biomass and BAP hormone on in vitro regeneration of medicinal herb (Hyssopus officinalis L.). Journal of Horticulture. 27: 207-201.
-  Jones A.M.P. and Sexena, P.K. 2013. Inhibition of phenylpropanoid biosynthesis in artemisia annua L.: A novel approach to reduce oxidative browning in plant tissue culture, PLoS One. (10): 1-13. doi: 10.1371/journal.pone.00768028.
-  Ghezelbash, S., Qaderi, A., Bodaghi, H., Ghasimi Hagh, Z., Kashefi, M. and Zare Karizi, A.R. 2016. The effect of antioxidant compounds and media on biosynthesis of limiter phenolic compounds during In vitro culture of Mentha arvensis L., Journal of Medicinal Plants, 16 (1): 1-9.
-  Khatibzadeh, R., Azizi, M., Arouiee, H. and Farsi, M. 2013. Effects of Sterilization Protocol and Pre-chilling Treatment on In vitro Seed Germination of Levisticum officinale Koch, Journal of Horticultural Science, 27( 2): 130-138. 
-  Krishna, H., Sairam, R.K., Singh, S.K., Patel, V.B., Sharma, R.R., Grover, M., Nain, L. and Sachdev, A. 2008. Mango explants browning: effect of ontogenic age, mycorrhization and pre-treatments. Science Horticulture Amsterdam, 118 (2): 132 - 8.
-  Lamber, T.J., Sirvastava, J., and Vietmeyer, N. 1997. Medicinal plants, rescuing a global heritage, Washington D. C., World Bank Technical Paper, 355
-  Quazi, M.H. 1980. In vitro multiplication of Lavandula spp. Annals of Botany, 45(3): 361-362.
-  Rechinger, K.H. 1982. Labiatae. In: “Flora Iranica, Browicz, K.H., K. Persson and P. Wendelbo (Eds.)”.
-  Akademische Druk-und Verlasantalt, Wiena, Graz, Austria, ISBN: 3-201 -00728-5, pp: 25-44.
-  Salehi, P., Sonboli, A., Eftekhar, F., Ebrahimi, Nejad, S. and Yousefzadi, M. 2005.Essenial oil composition antibacterial and antioxidant activity of the oil and various extracts of Ziziphora clinopodiodies sub sp. Rigida (BOISS). Journal of Biological and Pharmacetical Bulletin, 28 (10):1892-1896.
-  Sarwar, S., Zia, M., Riaz-Ur, R., Zarrin, F. and Riaz A. 2009. In vitro direct regeneration in mint from different explants on half strength MS medium. African, Journal of Biotechnology, 8(18): 4667-4671.
-  Shamsuddini, M., Pakneiyyat, H. and Niyazi, A. 2013. Micropropagation of different ecotypes of Zataria multiflora under different hormonal treatments. Master's Thesis. Shiraz University. 20-6.
-  Tabatabai, B. and Omidi, M., 2009. Plant tissue and cell culture. Tehran University Press
-  Taheri, A., Kowsari Nasab, M. and Movafeghi, A. 2014. Effect of Hormonal Treatments on Callus production in Ziziphora persica. The first national environmental conference. Tabriz. 7-1.