The effect of different factors on somatic embryogenesis of stevia plant (Stevia rebaudiana Bertoni.)

Document Type : Research Paper

Authors

1 M.Sc. student, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, I.R. Iran.

2 Assist. Prof., Department of Horticultural Science, Faculty of Agriculture & Natural Resources and Medicinal and Industrial Plants Institute, Ardakan University, Ardakan, I.R. Iran.

3 department of Soil Science , Natural Resources faculty, Yazd University

Abstract

DOR: 98.1000/1735-0891.1398.27.103.53.1.1575.41
Stevia plant(Stevia rebaudiana Bertoni.) is an important plant species due to its high content of recoverable natural sweeteners. Considering the industrial and medical importance of plant, the current study was performed with the aim of optimizing somatic embryogenic of this plant. Embryogenic callus induction and embryogenesis were investigated using different concentrations of Benzyl Adenine (BA), Naphthalene Acetic Acid (NAA) and 2,4-Dihydrophenoxy acetic acid (2,4-D) in leaf and bud explants in MS culture medium in a factorial experiment based on completely randomized design (CRD) with five replications. The highest callus induction was obtained in culture media contained 0.01 mg/l (BA + 0.5 mg/l 2, 4-D and the highest embryo induction was obtained in 0.01 mg/l BA + 1 mgl-1 2, 4-D treatment. Also the effect of Casein hydrolysate on the embryogenesis of the Stevia plant were investigated in five concentrations (0, 50, 100, 150 and 200 mgl-1) and coconut milk in two levels (0 and 55 ml) by both explants of leaf and bud in a factorial experiment based on CRD with six replications.  In concentrations of 0 and 50 mg/l of Casein hydrolysate respectively obtained the highest (20.2%) and lowest (7.84%) embryos. The percentages of embryogenesis in MS medium without coconut milk was 78.82% for bud explants and 25.09% for leaf explants, and finally, embryos were transferred to medium containing 0.1 mgl-1 of GA3 for organogenesis, and 18.03% of the embryos were grown to complete seedling.

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References

-  Aggarwal, A., Singh, N. and Yadav, K. 2011. Influence of arbuscular mycorrhizal (AM) fungi on survival and development of micropropagated Acorus calamus L. during acclimatization. International Journal of Agricultural Technology, 7(3): 775-781.
-  Altaf, T., Amin, S., Singh, S. and Kaloo, Z.A. 2013. Micropropagation of medicinally important plant species of family asteraceae– a review. International Journal of Recent Scientific Research, 4 (8): 1296- 1303.
-  Babber, S., Mittal, K., Ahlawat, R. and Varghese, T. 2001. Micropropagation of Cardiospermum halicacabum. Biologia Plantarum, 44: 603-606.
-  Bagheri, A., Moshiri, F. and Khosravinia, S. 2014. Investigation on reaction of explants and plant growth regulators on callus induction, rooting and in vitro regeneration of Bunium persicum (Boiss.) B. Fedtsch. Crop Biotechnology, 3(5): 53-61.
-  Banerjee, M. and Sarkar, P. 2010. Somatic embryogenesis in (stevia rebaudiana Bertoni) using different concentration of grow hormones. International journal of plant sciences, (5)1: 284- 289.
-  Das, A. and Mandal, N. 2010. Enhanced Development of Embryogenic Callus in (Stevia rebaudiana Bertoni). Biotecnology, 9(3): 368-372.
-  Din, M.S.U., Chowdhury, M.M.H. and Khan M.B.U. 2006.In vitro propagation of Stevia rebaudiana Bertoni in Bangladesh. African Journal of Biotechnology, 5: 1238-1240.
-  Gaj, M.D. 2004. Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to (Arabidopsis thaliana L.) Heynh. Plant Growth Regulation, 43: 27-47.
-  Gatica, M. A., Arrieta, G. and Espinoza, A. M. 2008. Direct somatic embryogenesis in (coffea arabica L.) cvs. Caturra and catua: effect of triacontanol, light condition, and medium consistency. Agronoma Costarricense, 32 (1): 139-147.
-  Gopi, C. and Rosemary, M.D. 2014. In vitro Plant Regeneration through Somatic Embryogenesis in Medicinally Important leaf explants of Coleus forskohlii Briq. Journal of Agriculture and Veterinary Science, 7 (9): 20-23.
-  Gram, T., Mattson, O. and Joerson, M. 1996. Division frequently of pea protoplast in relation to starch accumulation. Plant Cell Tissue and Organ Culture, 45: 3. 179-187.
-  Inocente, G.C.C., Vesco, L.L.D., Steinmacher, D., Torres, A.C. and Guerra, M.P. 2007. Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowian (Berg) Burret): Induction, conversion and synthetic seeds. Scientia Horticulture, 111: 228-234.
-  Keshvari, T. 2016. Improvement of Somatic embryogenesis in medicinal plant of Stevia. Ms.c thesis in Razi university. (In Persian).
-  Kumar, U.K. 1999. Methods in plants tissue culture. Agrobios. pp. 139- 147, India.
-  Loschiavo, F., Pitto, L., Giuliano, G., Torti, G., Nuit- Ronchi,V., Marazziti,D., Vergara, R., Orselli, S. and Terzi, M. 1989. DNA methylation of embryogenic carrot cell culture and its variation as caused by mutation differentiation hormones and hypomethyalating, Theory Apply Genetic, 77: 325-331.
-  Marks, T. and Simpson, S. 1990. Reduced phenolic oxidation at culture initiation in vitro following the exposure of field-grown stock plants to darkness or low levels of irradiance. Journal of Horticultural Science, 65: 103-111.
-  Monteuuis, O. and Bon, M.C. 1985. Microbuturage dusequoia geant. Ann Rech Sylvicoles, AFOCEL, Pp: 49-87.
-  Moradi, S., Azimi, M., Pourdad S.S and Habibi, F. 2017. Direct somatic embryogenesis from immature embryos of sunflower hybrids. Iranian Journal of Field Crop Science, 47(4): 701-707. DOI: 10.22059/ijfcs.2017.126609.653888. (In Persian).
-  Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3): 473-497.
-  Musavizadeh, S.J., Mashayekhi, K., Hemmati, Kh. and Kamkar, B. 2010. Evaluation of media elements and materials on petiole somatic embryogenesis of Carrot (Daucus carota L.). Journal of Plant Production, 17(1): 1-21. (In Persian).
-  Naranjo, J.E., Fernandez-Betin, O., Urrea-Trujillo, A.J., Callejas- Posada, R. and Atehortua-Garces, L. 2015.  Effect of genotype on the in vitro regeneration of (Stevia rebaudiana) via somatic embryogensis. Acta biologica Colombiana Journal, 21(1):87-98.
-  Nutironchi, V., Caligo, M.A., Nozzolini, M. and Luccarini, G. 1984. Stimulation of carrot somatic embryogenesis by proline and serine. Plant Cell Reports, 3: 210-214.
-  Pand, S. and Khetmalas, M. 2012. Effect of concentration of sucrose on callus induction & Somatic embryogenesis of anti-diabetic plant: stevia rebaudiana. Asian Journal of biochemical and pharmaceutical research, 1(2): 27- 31.
-  Piri Zirkouhi, M., Mashayekhi, K., Kamkar, B., Hemmati, Kh. and Vahdatpour, F. 2009. Embryogenesis of a commercial and a native tomato cultivar using different culture media, Journal of Plant Production Research, 16(1): 101-114. (In Persian)
-  Pola, S.R. and Srada, M.N. 2006. Somatic embryogenesis and plantlet regeneration in (Sorghum bicolor L.) Moench, from leaf segments. Journal of Cell & Molecular Biology, 5: 99- 107.
-  Sanjabi, M. 2016. Induction of direct somatic embryogenesis in leaf explants of Ulmus glabra. Journal of Plant Researches, 28(4): 885-894.
-  Sekaran, T., Giridhar, P., Ravishankar, G.A. 2007. Production of steviosides in ex vitro and in vitro grown Stevia rebadiana Bertoni. Journal of Science Food & Agriculture, 87: 420-424.
-  Thom, M., Maretzki, A., Komor, E. and Sakai, W.S. 1981. Nutrient uptake and accumulation by sugarcane cell cultures in relation to the growth cycle, Plant Cell Tissue and Organ Culture, 1: 3-14.
-  Weatherhead, M., Burdon, J. and Henshaw, G. 1979. Effects of activated charcoal as an additive to plant tissue culture media: Part 2. Z Pflanzenphysiol, 94: 399-405.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 27, Issue 1 - Serial Number 53
September 2019
Pages 108-119

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