Micropropagation of Eucalyptus citriodora H.

Document Type : Research Paper

Authors

1 MSc, Expert of Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.

2 Professor, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran

3 Assistant professor, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran

Abstract

DOR: 98.1000/1735-0891.1398.27.88.53.1.32.97
Among species of hardwood forest trees, Eucalyptus citriodora is a fast growing plant that is cultivated for aromatic essential oils in perfumes, pharmaceuticals, and its soft and light wood in the paper and furniture industry. The micropropagation of E. citriodora Lemon-scented Gum was carried out in two medium culture of MS (1/2N) and WPM through bud culture. Sterilization was done using solutions of mercuric chloride 0.1% and sodium hypochlorite 20%. Shoot multiplication was done with cytokinin (BAP, Kin), auxin (IBA) and gibberellin (GA3) hormones and rooting with auxin hormones IBA, NAA and IAA in only MS (1/2N) medium. The shoot multiplication was assessed in two medium using factorial experiment based on the completely randomized design (CRD) and rooting multiplication in one medium using CRD. Propagation coefficient, shoot length, greenity, total root and rooted seedlings were measured. The best sterilization was immersion seeds in 20 % sodium hypoclorite solution in 18 min. The best propagation and shoot elongation was obtained in MS (1/2N) medium coupled with BAP, Kin, GA3 cytokinin hormones and auxin hormones IBA in 0.3, 0.2, 0.1, 0.01 mgl-1 and 0.3, 0, 0.1, 0.01 mgl-1concentrations respectively by 200 mgl-1 P.V.P. The best rooting treatments were a modified MS (1/2N) medium, auxin hormone NAA and IBA+IAA in 1 mgl-1, 0.5mgl-1. These plantlets transferred to soil and were kept in the greenhouse conditions. The results of this study show that tissue culture is a suitable method for the propagation of this valuable species in a short time.

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Main Subjects

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 27, Issue 1 - Serial Number 53
September 2019
Pages 86-97

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