The study of genetic diversity of cumin ecotypes of Khorasan provinces using protein markers

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Birjand

2 Assoc. Prof., Department of Agronomy and Plant Breeding, College of Agriculture, University of Birjand, I.R.Iran

3 Assist. Prof., Department of Agronomy and Plant Breeding, College of Agriculture, University of Birjand, I.R.Iran

Abstract

DOR: 98.1000/1735-0891.1397.2.292.52.26.1576.1610

Cuminum cyminum is one of the oldest and most economically important species in the
Apiaceae family that has the highest cultivated areas of Khorasan provinces in Iran. Genetic
diversity of 20 ecotypes of Cuminum cyminum was investigated by protein markers. The
ecotypes were randomly collected from three provinces of North, Razavi and South Khorasan
provinces. Protein profile analysis was carried out by SDS-PAGE method. The method
included: extracting protein from the sample seeds, determining samples concentration, and
finally running on electrophoresis gel. Analysis of molecular variance showed that there was
variation between and within the populations. Based on the protein markers %24 of the
variations belonged to between populations and %76 belonged to within populations.
Polymorphism percentage, polymorphic content mean, Shannon's information index and the
average of Nei's information index were 27%, 0.01, 0.49, and 0.68, respectively. Results of
PCoA showed that the first three components accounted for 83% of the variation. Cluster
analysis based on the similarity matrix classified the studied ecotypes in 5 clusters.

Keywords

References

-  Anderson, J.A., Churchill, G.A., Sutrique, J.E., Tanksley, S.D. and Sorrells, M.E., 1993. Optimizing parental selection for genetic linkage maps. Genome Biology, 36:181-186.
-  Bagheri, A.A. and Mahmoodi, A.A., 2002 Cumin production and processing technology Department of Agronomy, Faculty of Agriculture, Ferdowsi University Press 143-150.
-  Bihamta, M.R., Naserian Khiabani, B. and Zamani, M.J., 2009. Molecular markers in plant genetics and biotechnology (translation). Tehran University Press , P 53-54.
-  Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annual Biochemistry, 72: 248-254.
-  Farooq, F. and Azam, F., 2002. Molecular markers in plant breeding: concepts and characterization. Pakistan Journal of Biological Science, 5(10): 1135-1140.
-  Farshadfar, A., 2010. Molecular Plant Breeding. Taghe Bostan Publication, Kermanshah, Iran.
-  Guo, L.J., 2013. Molecular markers and marker-assisted breeding in plants. Intech Open Science/ Open Minds http:// creative commonsorg/licenses/by/30.
-  Kafi, M., 2001. Cumin ecophysiology Cumin article series technology production and processing Ferdowsi University Press, Mashhad, I.R.Iran.
-  Khalilipour, J., 2009. Green cumin. Skill Monthly Journal of Scientific Technical and Profession 50: 38-39.
-  Kim, S.T., Cho, K.S., Jang, Y.S., and Kang, K.Y., 2001. Two-dimensional electrophoretic analysis of rice proteins by polyethylene glycol fractionation for protein arrays. Electrophoresis, 22: 2103-2109.
-  Kimura, M. and Crow, J.F., 1963. The measurement of effective population number. Evolution, 17: 279-288.
-  Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259): 680-685.
-  Lewontin, R.C., 1972. The apportionment of human diversity. Evolution Biology, 6: 381–398.
-  Lynch, M., and Milligan, B., 1994. Analysis of population-genetic structure using RAPD markers. Molecular Ecology 3: 91-99.
-  Magni, C., Scarafoni, A., Herndi, A., Sessa, F., Prinsi, B., Espen, I., and Duranti, M., 2007. Combined 2D electrophoretic approaches for the study of white Lupine mature seed storage proteome. Phytochemistry 68: 997-1007.
-  Masoumi, S.M., Kahrizi, D., Rostami- Ahmadvandi, H., Soreni, J., Kiani, S., Mostafaie, A. and Yari, K.H., 2012. Genetic diversity study of some medicinal plant accessions belong to Apiaceae family based on seed storage proteins patterns. Molecular Biology Reports, 39:10361-10365.
-  Masoumi, S.M., Kahrizi, D., Soreni, J., Rostami, H., Mostafaie, A. and Kiani, S., 2011. Study of genetic variation of some cumin mass based on seed storage (Cuminum cyminum) proteins by SDS- PAGE. Annual scientific conference of Razi University from December 20 to December 23, 2011.
-  Nei, M., 1987. Molecular Evolutionary Genetics. New York: Columbia University Press.
-  Nei, M. and Li, W.H., 1979 Mathematical model for studying genetic variation in terms of restriction endonucleases. Journal of Proceedings of the Natural Academy of Science of the USA, 76:5269-5273.
-  Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalsky, A., 1996. The comparison of RFLP RAPD AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238.
-  Rahmanpour, S., Abdollahi Mandolkani, B. and Ghadimzadeh, M., 2014. Evaluation of genetic variation of native and hybrid Melon ecotypes using ISSR markers. Journal of Genetics, 9 (1): 76-67
-  Ravi, M., Geethanjali, S.F., Sameeyafarheen, F.M. and Maheswaran, M., 2003. Molecular marker based genetic diversity analysis in rice (Oryza sativa L) using RAPD and SSR markers. Euphytica, 133:243–252.
-  Rostami-Ahmadvandi, H., Cheghamirza, K., Kahrizi, D. and Bahraminejad, S., 2013. Comparison of morpho-agronomic traits versus RAPD and ISSR markers in order to evaluate genetic diversity among Cuminum cyminum L accessions. Australian Journal of Crop Science, 7(3):367-361.
-  Shorideh, H. and Pekniyat, H., 2004. Study of genetic diversity of Cumin (Cuminum Cyminum) ecotypes of Iran using seed proteins. Electrophoresis Proceedings of the First National Symposium on Cumin, Sabzevar, Iran P 109.
-  Soreni, J., Rostami Ahmadvandi, H., Kahrizi, D., and Masoumi, S.M., 2012. Investigation of the Relationship between Protein and Morphological markers in Cuminum Cyminum L. 12th Iranian Congress of Genetics, Tehran, May 3-1.
-  Taheri, S., Zabet, M., Izanlo, A. and Izadi Darbandi, A., 2016. Assessment of genetic diversity of fennel ecotypes using RAPD and ISSR markers. Journal of Agricultural Biotechnology, 7 (4): 113-128.
-  Uzun, A. and Yesiloglu, T., 2012. Genetic diversity in Citrus In: Genetic Diversity in Plants, Calhskan, M. (Ed) In Tech, PP: 213-231.
-  Wright, S., 1951. The genetical structure of populations. Annuals of European Genetics, 15: 323-354.
-  Zakeri, M.A., Salehi Shanjani, P., Javadi, H., and Alizadeh, A., 2014. Genetic variation of A. triumfetti, A pseudocutula and Anthemis haussknechtti species using electrophoretic pattern of total proteins. Iranian Journal of Rangelands Forests Plant Breeding and Genetic Research, 22 (2): 302-311.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 26, Issue 2 - Serial Number 52
December 2018
Pages 292-301

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