Genetic variability of Tanacetum polycephalum populations in West Azarbaijan using ISSR molecular markers

Document Type : Research Paper

Authors

1 PhD Student, Department of Biology, Faculty of Science, Urmia University Pardis, Urmia University, Urmia, I.R. Iran. Email: Mojarrad2017@gmail.com

2 Corresponding author, Assist. Prof, Department of Biology, Faculty of Science, Urmia University, Urmia, I.R. Iran.

3 Assoc. Prof., Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, I.R. Iran.

4 Prof., Saba Nonprofit University, Urmia, I.R. Iran.

Abstract

DOR: 98.1000/1735-0891.1397.2.268.52.26.1576.82

Tanacetum polycephalum from Anthemideae tribe of Asteraceae family is a rangeland
species with high morphological diversity and vast dispersal in Northwest, East and centre parts
of Iran. The purpose of this research was studying genetic diversity of 20 populations of T.
polycephalum in West Azarbayjan using ISSR molecular marker. Ten ISSR primers out of 14
primers could be scored based on presence or absence of each band. Over all 208 bands were
produced .The range number of bands was between 15(IS24) to 28 (IS20) and the average was
20.8. Regarding the polymorphism bands percentage, IS8, IS1, IS10, IS13, IS20 markers had
high percentage (100) and IS24 marker had the lower percentage (80) and mean percentage of
polymorphism of the primers was %96.03. PIC, RP, EMR and MI means were 0.30, 6.06, 19.5
and 6.13 respectively. Over all the most effective markers were IS8, IS1 and IS10. Results of
cluster analysis revealed the grouping of studied populations in 2 main and 4 subgroups, in
which the different populations collected from the same locality were located in the same group.
Therefore, a close relationship was characterized between geographic location and genetic
diversity and it may be concluded that geographic and climatic factors could be one of the
responsible main factor for genetic diversity.

Keywords

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 26, Issue 2 - Serial Number 52
December 2018
Pages 268-278

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