Evaluation of genetic diversity effects on morpho-physiological and antioxidant responses in different species of Aegilops under drought stress

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Production and Plant Breeding, Imam Khomeini International University, Qazvin, Iran

2 M.Sc. Student, Imam Khomeini International University, Qazvin, I.R.Iran

Abstract

DOR: 98.1000/1735-0891.1397.2.254.52.26.1605.1610

This experiment was conducted to investigate the effect of drought stress on photosynthetic
pigments contents, biomass of roots and shoots, total protein content and antioxidant enzymes
activity and non-enzymatic antioxidant of six species of Aegilops genus along with two drought
resistant and susceptible wheat cultivars as checks in a factorial based on randomized complete
block design with three replications. Drought stress treatments were applied at three levels of
non-stress (FC=100%), moderate stress (FC=50%) and severe stress (FC=25%) conditions. Ae.
caudata, Ae. triuncialis and Ae. cylindrica were ranked higher than resistance cultivar, Sirvan,
as check in term of root fresh and dry weights under stresses. The highest total chlorophyll
content was related to Ae. cylindrica, Ae. umbellulata and Ae. caudata in stresses. Carotenoid
showed decreasing trend in all studied species except for Ae. umbellulata, and Ae cylindrica had
the highest amount at both stress and non- stress conditions. The highest SOD under severe
stress was observed in Ae. crassa and Sirvan as resistant cultivar. Maximum APX under severe
stress was observed in Ae. triuncialis and Ae. caudata and the lowest activity was in Ae.
neglecta. The highest level of GPX was observed in Ae. triuncialis, Ae. caudata and Sirvan
cultivar under drought stress. Also the maximum CAT enzyme under severe stress was
observed in Ae. triuncialis, Ae. caudata and Ae. crassa, respectively, and Ae. umbellulata and
Darya cultivar had the lowest amount of CAT enzyme. In conclusion, because the three species
Ae. caudata, Ae. triuncialis and Ae. cylindrica had the highest amounts for the traits associated
with increased drought tolerance, including root volume, carotenoid, CAT, GPX and APX
enzymes, and also these species were same in having CC genome. Therefore, it seems that the
CC genome could have a high potential for future wheat breeding programs in increasing of
drought tolerance.

Keywords

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 26, Issue 2 - Serial Number 52
December 2018
Pages 254-267

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