Tissue culture and organogenesis of Iranian mountain ash (Sorbus persica Hedl.)

Document Type : Research Paper

Authors

1 Corresponding author, Assist Prof., Research Division of Natural Resources, Isfahan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization, AREEO, Isfahan, I.R.Iran

2 Assoc. Prof. Department of Forestry, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, I.R. Iran.

3 3-Assoc. Prof., Biotechnology Research Division, Research Institute of Forests and Rangelands, Education and Extension Organization, AREEO, Tehran, I.R. Iran.

4 Assoc. Prof., Biotechnology Department, College of Agriculture, Isfahan University of Technology, Isfahan, I.R. Iran.

Abstract

DOR: 98.1000/1735-0891.1397.2.233.52.26.1605.32

Iranian mountain ash (Sorbus persica Hedl.) is one of the endangered species with great
values in terms of soil conservation, climate stratification, medicinal properties, high resistance
to frost, and importance of habitat for wildlife. Present research was conducted with the aim of
achieving to an appropriate method of micropropagation of the species. Nodal segments were
taken from stem axis of in vitro elongated plants. The segments were cultured on WPM
medium, supplemented with 27 hormonal composition of BAP, IBA and TDZ. Then the
resulting shoots were transferred to a media supplemented with various concentrations of IBA
or NAA for rooting induction, and finally they were moved to a greenhouse. Percentages of
regeneration of the explants were 98 -100%. The highest number of shoots was obtained by
TDZ (0.23 μM) + IBA (0.1 μM) and BA (4.4 μM), the highest shoot length (5.2 cm) was
obtained by TDZ (0.0 μM) + IBA (0.0 μM) and BA (4.4 μM) and the highest number of nodes
(8) was achieved by TDZ (0.23 μM) + IBA (0.0 and 0.1 μM) and BA (2.2 and 4.4 μM). There
was a significant difference between IBA concentrations in different treatments for rooting
percentage. Maximum rooting percentage was 14.7% in IBA concentration of 5 μm. The rooted
segments were successfully acclimated to greenhouse conditions. Results of the experiment
showed that in the absence of BAP hormone, umber of shoots produced by the segments
significantly were decreased for all of the tested treatments. Fifteen percent of the transferred
explants to the rooting media produced one to three roots per explant.

Keywords

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 26, Issue 2 - Serial Number 52
December 2018
Pages 233-243

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