Investigating morphological characteristics and genetic relationships between two populations of Ferula pseudalliacea in Yazd Province

Document Type : Research Paper

Authors

1 Corresponding author, PhD Student, Rangeland Science, Department of Natural Resources, Agricultural Science and Natural Resources University of Gorgan, Gorgan, I.R. Iran. Email: Samirahosseinjafari@yahoo.com

2 Prof., Department of Range Management, Faculty of Natural Resources, Agricultural Science and Natural Resources University of Gorgan, Gorgan, I.R. Iran.

3 Associ. Prof., Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Agricultural Science and Natural Resources University of Gorgan, Gorgan, I.R. Iran.

4 Associ. Prof., Department of Range and Water Management, Faculty of Natural Resources and Desert Study, Yazd University, Yazd, I. R. Iran.

Abstract

DOR: 98.1000/1735-0891.1397.2.221.52.26.1576.32
Ferula pseudalliacea (bitter assafoetida) is a rangeland plant which its gum has a lot of
medicinal properties. Since there is no report about evaluating genetic diversity of the valuable
species in Iran, this is the first study to investigate genetic diversity of the species in its habitats
in Yazd province using several morphological traits and ISSR markers. Among 9 traits, there
was significant differences between habitats in terms of canopy cover, stem diameter and weight
of 1000 seed. Cluster analysis by morphological traits on the basis of Ward's method classified
the samples into three main groups. Among 22 primers, 8 primers reproduced the pattern DNA
well and revealed 224 sharp bands. ISSR-16 and ISSR-55 primers had better performance
according to the number of bands, PIC and Marker Index. Cluster analysis using Jacquard's
coefficient and UPGMA method, grouped two populations into 10 clusters. Principal
coordinates analysis showed three components explained 57.94 percent of total variance and
separated the populations from each other. These grouping matched relatively with the
geographical regions. AMOVA showed that within group genetic diversity (92%) was more
than between group diversity (8%). Comparing morphological and molecular dendrograms
revealed that there was no similarity between them. The results showed that ISSR marker was
suitable to investigate genetic diversity among the studied populations of the species.

Keywords

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 26, Issue 2 - Serial Number 52
December 2018
Pages 221-232

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