Application of SCoT marker to discriminate Lolium perenne and Lolium multiflorum species

Document Type : Research Paper

Authors

1 Corresponding author, Assoc. Prof., Department of Agriculture, Payame Noor University, I.R. Iran Email: farshadfarmohsen@yahoo.com

2 Instructor, Department of Agriculture, Payame Noor University, I.R. Iran

3 Department of Agriculture, Payame Noor University, I.R. Iran

4 Ph.D in Plant breeding, Department of Agronomy and Plant breeding, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, I.R. Iran

Abstract

DOR: 98.1000/1735-0891.1397.2.207.52.26.1603.109

Lolium is an important cool season grass species growing in different climates. To identify
variation among nine Lolium multiflorum and nine Lolium perenne genotypes, this experiment
was carried out based on 15 SCoT molecular markers. All of the SCoT primers showed 86
visible bands. Seventy four out of 86 bands were polymorphs. Primers SC35, SC36 and SC26
(with 9 and 8 bands respectively) showed the highest number of bands and SC10 and SC44
(with 2 and 3 bands) showed the lowest number of bands. The greatest number of band for
polymorphic information content (PIC) belonged to SC5، SC40، SC35 ،SC63 ،SC13, C44،
SC26 ،SC21and SC10. The greatest Resolving Power (RP), Marker Index (MI), Effective
Multiplex Ratio (EMR) belonged to SC35 andSC26 primers. Genetic similarity based on
Jaccard coefficient ranged from 0.35 to 0.91. Mean similarity between the genotypes was 0.64.
Cluster analysis grouped genotypes into two groups. The results of clustering was confirmed by
principal coordinate (PCo) analysis. Analysis of molecular variance of annual and perennial
species revealed that variation within species was 51% and variation between species was 49%.
Based on heterogeneity (He) and Shannon indices (I), the highest intraspecific variability and
gene variation belonged to L. Multiflorum.

Keywords

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 26, Issue 2 - Serial Number 52
December 2018
Pages 207-220

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