Genetic diversity of several Iranian sumac (Rhus coriaria) populations using genomic inter-microsatellites markers

Document Type : Research Paper

Authors

1 M.Sc., Plant Breeding, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, I. R. Iran.

2 Corresponding author, Prof., Department of Plant Breeding and Biotechnology, Urmia University, Urmia, I.R. Iran. Email: r.darvishzadeh@urmia.ac.ir

3 Assoc. Prof., Department of Forestry, Faculty of Natural Resources, Urmia University, Urmia, I. R. Iran.

4 Assist. Prof., Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, I. R. Iran.

5 M.Sc., Agricultural Biotechnology, Institute of Biotechnology, Urmia University, Urmia, I. R. Iran.

Abstract

DOR: 98.1000/1735-0891.1397.2.196.52.26.1576.82

Sumac (Rhus coriaria L.) as a forest shrub has been dispersed in Mediterranean and Eastern
Asia specially Iran and has several medicinal and industrial applications. In this research, intermicrosatellites
markers were used to study genetic diversity of sumac genotypes collected from
five natural habitats located in North West of Iran (West and East Azerbaijan provinces).
Fifteen samples were select randomly from each population and genomic DNA was
fingerprinted by 18 ISSR markers. Results revealed existence of suitable genetic variation
among the studied sumac genotypes and portion of inter population (79.8%) variation were
higher than intra population (20.2%) variation. Minimum value of genetic distance (14.18) was
observed between Aghberaz-Horand (East Azerbaijan province) and Nir-Arasbaran (East
Azerbaijan province) populations and the maximum one (31.08) was observed between
Kachelleh-Urmia (West Azerbaijan) and Nir-Arasbaran populations. According to classification
of genotypes based on molecular data, Aghberaz-Horand and Nir-Arasbaran populations located
in the same group and populations from other habits located in distinguished groups.
Classification of genotypes via ISSR markers was in agreement with their geographical
distributions. ISSR markers could be effectively applicable in genetic evaluation of sumac
germplasm and also in identification of genetic relatedness among known and unknown sumac
samples.

Keywords

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 26, Issue 2 - Serial Number 52
December 2018
Pages 196-206

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