Identification of miRNAs and their target genes in red clover (Trifolium pretense)

Document Type : Research Paper

Authors

1 Corresponding author, Prof., College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R.Iran Email: mnaghavi@ut.ac.ir

2 M.Sc. student, College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R.Iran

Abstract

DOR: 98.1000/1735-0891.1397.2.155.52.26.1588.41

MicroRNAs (miRNAs) are a group of 18_22 nucleotides long noncoding small endogenous
that derived from its precursor sequence and evolutionary conserved post-transcriptional
regulatory RNAs, which show an enormous role in various biological and metabolic processes
in both animals and plants. MiRNA detection methods including microarray, qRT-PCR ,
northern blot and bioinformatics methods in which the easiest and cheapest way to identify
miRNA is bioinformatics method. A bioinformatics approach was used to identify potential
miRNA in red clover. EST-based homology search was applied to find potential miRNA of red
clover. We blasted publicly available EST sequences obtained from NCBI GenBank against
previously known plant miRNAs. A total of six miRNA target genes based on their
complementary sequences were also detected. Target genes encoding mono dehydro ascorbate
reductase 1 play an important role in maintaining balance in the cycle of reactive oxygen
species ascorbate-glutathione, FES1 proteins that contain zinc finger domain binding DNA,
RHF2A gene encoding E3 ubiquitin-protein ligase involved in the positive regulation of the
gametogenesis progression, RNA helicase ATP-dependent is involved in the RNA repair and
metabolism, Major facilitator superfamily (MFS) is a superfamily of membrane transport
proteins that facilitate movement of small solutes across cell membranes in response to
chemiosmotic gradients, AT-hook DNA binding motif protein.

Keywords


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