Effects of different growth regulators, gelling agents and carbon sources on in vitro growth of Berberis vulgaris var. asperma

Authors

1 Corresponding author, Associate Prof., Fars Research and Education Centre for Agriculture and Natural Resources, Shiraz, I.R.Iran E-mail: y.saadat1336@gmail.com

2 B.Sc. Fars Research and Education Centre for Agriculture and Natural Resources, Shiraz, I.R.Iran

Abstract

  Berberis vulgaris var. asperma, is a medicinal plant of Iran and lack of healthy vigorous
seedlings is the main limitation of barberry cultivation. This research was carried out to develop
a suitable technique for mass micropropagation of the species. Effects of different plant growth
regulators, different gelling agents and different carbon sources on in vitro growth of  barberry
were studied in several experiments. Based on the results of the experiments, nodal segments
containing 1-2 axillary buds of semi-hardwood shoots were the best explants for establishment
of clean in vitro cultures. Surface disinfection of plant materials originated from adult trees was
performed with immersion in 2 gl
-1
 of Benomyl solution for one hour, then 1% solution of
commercial bleach for 15 minutes and rinsed three times with sterile distilled water. Explants
cultured on media solidified with Phytagel were significantly better than the media with
DifcoBacto agar and Agargel for shoot production. Addition of 3 gl
-1
 activated charcoal resulted
in control of nutrient medium and explants browning, improvement of survival and in vitro
growth of the explants. DKW medium containing 2 mgl
3 gl
-1
 activated charcoal and solidified with 2.5 gl
-1
-1
 BA, 0.5 mgl
-1
 IBA , 2 mgl
 Phytagel was optimum for in vitro growth
of barberry and recommended for shoot multiplication of the species. Rooting experiment was
carried out using a two phase procedure, root induction in media with high concentration of
IBA, followed by transferring to growth regulators free medium for root development. No
rooting was observed and further research is needed.
 

Keywords

References

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