Effect of juvenile tissue and plant growth regulators on in vitro shoot proliferation of Pronus avium L.

Authors

1 Corresponding author, Assoc. Prof., Agriculture and Natural Resources Research Centre of Gorgan, Agricultural Research, Education and Extension Organization (AREEO), Gorgan, I.R. Iran. Email: jafarimofidabadi@gmail.com

2 M.Sc., Agriculture and Natural Resources Research Centre of Gorgan, Agricultural Research, Education and Extension Organization (AREEO), Gorgan, I.R. Iran.

3 -M.Sc., Agriculture and Natural Resources Research Centre of Gorgan, Agricultural Research, Education and Extension Organization (AREEO), Gorgan, I.R. Iran.

Abstract

 Propagation of Pronus avium L. through conventional micropropagation is essential for forest
development and production of scion for grafting of commercial Pronus varieties. For
micropropagation and production of juvenile tissue as an initial source of proliferation, mature
embryo explants were used. For in vitro shoots preparation, isolated embryos of mature trees
were transferred into MS medium containing 30 and 60 g/l sucrose. There were no significant
differences between MS media containing 30 and 60g/l for percentage of embryo germination
using t-Student test. Factorial analysis of collected data for further shoot proliferation indicated
that there were highly significant differences between the used growth regulators for in vitro
shoot length and number of produced explants‎at‎α=0.01‎level. The highest average shoot length
(5.86 cm) and the highest mean number of explants production were observed on MS medium
supplemented with 4 mg/l BA and 0.2 mg/l IBA. Plantlet at 4 to 5 cm in height were rooted in
MS hormone free medium and after successful acclimatization transferred to a green-house.

Keywords

References

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