Asexual regeneration of (Zhumeria majdae L.) by Bud culture

Authors

1 Corresponding author, Assist. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran. Email: emam@rifr-ac.ir

2 M.Sc., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran.

3 Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO),Tehran, I.R. Iran.

4 Assist. Prof., Department of Forest, Agricultural and Natural Resources Research Center of Hormozgan, Agricultural Research, Education and Extension Organization (AREEO), Bandarabbas, I.R. Iran.

Abstract

Zhumeria majdae is one of the most important aromatic plant species from Lamiaceae family. The valuable species has medicinal and industrial applications. It is an endemic and endangered species of Iran. Therefore, tissue culture techniques are proposed for propagation and conservation purposes of the species. Usual regeneration of the species is by seed. Apical buds of seedlings from Hormozgan genotypes were sterilized by HgCl2 0.1% solution for 5 minutes during spring season. The best medium for shoot proliferation was WPM containing BA (0.5 mg/l), 2iP (0.3) and IBA (0.1 mg/l). The highest rooting of the shoots was obtained by WPM supplemented with 1 mg/l of IBA. The plants were acclimatized in greenhouse after transferring in soil. The protocol of this research could be used for culture and developmental programs of the species in its original habitat.

Keywords

References

-  Aghazadeh, P. and Shahsavan Behboodi, B., 2015. Seed germination and in vitro organogenesis optimization of Zhumeria majdae (Lamiaceae) from Iran. American Journal of Plant Sciences, 6 (11): 1850-1856.
-  Akcamoluk. E., Çakir, A., Yasa, I., Çapanlar, S. and Kirmizigul, S., 2013. Comparison of the antimicrobial activity and essential oil content of wild and micro propagated Origanum sipyleum L.: A medicinal herb native to Turkey. Journal of Medicinal Plant Research, 7(6): 230-233.
-  Bonga, J.M. and Von Aderkas, P., 1992. In vitro culture of trees. In: Forestry Science Book, Vol. 38, Kluwer Academic Publishers, London, 236 p.
-  Chalupa,V., 1987. Effect of benzyl amino purine and thydiazuron on in vitro shoot proliferation of Tilia cordata Mill, Sorbus aucuparia L. and Robinia pseudo acaciae L. Biologia Plantarum, 29: 425-429.
-  Dalal, N.V. and Rai, V.R., 2004. In vitro propagation of Oroxylum indicum Vent. A medicinally important forest tree. Journal for Research. 9: 61-65.
-  Emam, M., Izadpanah, M., Jaffari Mofidabadi, A., 1990. Effect of Medium, Hormones and Genotypes on micropropagation of Populus caspica. Iranian Journal of Forest and Rangelands Plant breeding and Genetic Research, 2: 81-99.
-  Emam, M., Ghamari zare, A., Espahbodi, K., Naraghi, T.S., Shahrzad, Sh., 2001. Micropropagation of Sorbus aucoparia by Bud culture of mature trees.  Iranian Journal of Forest and Rangelands Plant breeding and Genetic Research, 19 (2): 263-273.
-  Emam, M., Ghamari zare, A., Asadi korom, F., Looki anaraki,K., 2003. Micropropagation of Amygdalus scoparia by Bud and embryo culture.  Iranian Journal of Forest and Rangelands Plant breeding and Genetic Research, 21 (1):77-86.
-  Enjarlic, F. and Lardet, L., 1988. Contamination of primary culture in tropical areas. Acta Horticulturae, 225: 57-65.
-  Evers, P.W., Donkers, J., Prat, A. and Vermeer, E., 1988. Micropropagation of forest trees through tissue culture: 98-102. In: Bonga, J.M., Aderkas. P., (Eds). In Vitro Culture of Trees. Centre for Agricultural Publishing and Documentation (pudoc): 236 P.
-  Murashige, T. and Skooge, F., 1962. A revised medium for rapid growth and bio- assays with tobacco tissue culture. Physiologia Plantarum, 15: 473-597.
-  Omidpanah, N., Asrar, Z. and Moradshahi, A., 2011. Investigation of allelopatic potencial of medicinal plant Zhumeria majdae on Kolza genotype. Plant Biology, 3(7): 1-10.
-  Ozudogru, E.A.  Kaya E.  Kirdok, E. and Ozturk. S. I., 2011. In vitro propagation from young and mature explants of thyme (Thymus volgaris and T. longicaulis) resulting in genetically stable shoots. In vitro Cell Development Biological Plant, 47: 309-320.
-  Sáez, F., Sanchez, P. and Piqueras. A., 1994. Micro propagation of Thymus piperella. Plant Cell Tissue and Organ Culture, 39: 269–272.
-  Soltanipoor, M.A., Moradshahi, A., Rezaee, M.B., Kholdebarin, B. and Barazande. M.M., 2006. Allelopaty effects of of Zhumeria essential on seed germination and seed growth of herbal plants, Lycopersicum and Wheat. Journal of Iran Biology, 19(1): 11-20.
-  Vincent, K.A., Mathew, K. M. and Hariharan, M., 1992. Micro propagation of Kaemferia galanga L. A medicinal plant. Cell Tissue and Organ Culture, 28: 229–230.
-  Yavarian, M.and Habibi., A. R, 2013. In vitro culture of plant extinction of Zhumeria majdae .Genetics.

Files

Share

How to cite

Statistics