Effective factors on micropropagation of medicinal plant of Satureja khuzistanica

Authors

1 Ph.D. student, College of Biological Sciences, Kharazmi University, Tehran, I.R. Iran.

2 Corresponding author, Assoc. Prof., College of Biological Sciences, Kharazmi University, Tehran, I.R. Iran,

3 Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran.

4 Accis. Prof., National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, I.R.Iran.

5 Accis. Prof., Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, I.R. Iran.

Abstract

Satureja khuzistanica Jamzad is an endemic species of Iran. The species is an endangered and valuable medicinal plant. In order to propagate through micropropagation, lateral buds were collected from Lorestan province, region of Pol of Dokhtar. The best treatment of sterilization of the explants was 0.1% HgCl2 for 6 minutes. The best medium for shoot regeneration was MS with BA, 2iP and IBA 0.3, 0.3 and 1 mg/l, respectively. Applying 12 different treatments, the mentioned medium was better than other treatments in term of the coefficient of elongation, leaf elongation and leaf greenness. The best medium for root regeneration was MS with 1 mg/l IBA and 0.1 mg/l NAA. It initiated the highest number of primary and secondary roots. Various combinations of different soils were evaluated for survival during the acclimatization, and sand: soil: peat: perlite at a ratio of 1: 1: 1: 1 showed the highest percentage of survival and growth. The best propagation method of the species was developed and the propagated plants could be transplanted into the habitat of the species.

Keywords

References

-  Ahmadvand, H., Tavafi, M. and Khalatbary, A.R., 2012. Hepatoprotective and hypolipidemic effects of Satureja khuzestanica essential oil in alloxan-induced type 1 diabetic rats. Iranian Journal of Pharmaceutical Research, 11: 1219–1226.
-  Amanlou, M., Dadkhah, F., Salehnia, A. and Farsam, H., 2005. An anti-inflammatory and anti-nociceptive effects of hydroalcoholic extract of Satureja khuzestanica Jamzad extract. The Journal of Pharmacy and Pharmaceutical Sciences, 8: 102–106.
-  Arrebola, A.M., Socorro, O., Barcelo-Monoz, A., Simon perez, E. and Fernando Perpliago A., 1997. Micropropagation of Satureja obavata Lag. Hort Sciences, 32 (7): 1278-1280
-  Driver, J.A. and Kuniyuki, A.H., 1984. In vitro propagation of Paradox walnut rootstocks (Juglans hindsii × J. regia). Horticultural Science, 19: 507-509.
-  Emam, M., Ghamarizare, A., Espahbodi, K., Naraghi, T.S., Shahrzad, Sh., Zare, H. and Mirjani, L., 2013. Effect of medium, plant growth regulators and genotype on In vitro regeneration of Sorbus aucuparia L. Iranian Journal of Medicinal and Aromatic Plants, 29: 85-95.
-  Jalili, A. and Jamzad, Z. 1999. Red data book of Iran. Research Institute of Forests and Rangelands. Tehran, Iran. 748 p.
-  Jamzad, Z., 2011. Thyme and Savory Iran. Research Institute of Forests and Rangelands, Tehran, 171 p.
-  Kaeidi, A., Esmaeili-Mahani, S., Abbasnejad, M., Sheibani, V., Rasoulian, B., Hajializadeh, Z. and Pasban-Aliabadi, H., 2013. Satureja khuzestanica attenuates apoptosis in hyperglycemic PC12 cells and spinal cord of diabetic rats. Journal of Natural Medicines, 67: 61–69.
-  Lloyd, G. and McCown, B.H., 1981. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by shoot tip culture. Combined Proceedings, International Plant Propagators' Society, 30:421–427
-  McCown, B.H. and Sellmer, J.C., 1987. Media and physical environment: 4-17. In: Bonga, J.M. and Durzan, D.J., (Eds.). Cell and Tissue Culture in Forestry (Vol 1), General principles and Biotechnology. Martinus Nijhoff Publishers, Pordrecht, 422p
-  Murashige, T. and Skooge, F., 1962. A revised medium for rapid growth and bio-assays with tobacco tissue culture. Physiologia Plantarum, 15: 473-597.
-  Motaharinia, Y., Hazhir, M.S., Rezaee, M.A., Vahedi, S., Rashidi, A., Hosseini, W., Hakhamaneshi, M.S. and Rahmani, M.R., 2012. Comparison of in vitro antimicrobial effect of ethanol extracts of Satureja khuzestanica, Rhus coriaria and Ocimum basilicum L. on Helicobacter pylori. Journal of Medicinal Plants Research, 6 (21): 3749–3753.
-  Mozafari, A.K., Vafaee, Y. and Karami, E., 2015. In vitro propagation and conservation of Satureja avromanica Maroofi an indigenous threatened medicinal plant of Iran. Physiology and Molecular Biology of Plants, 21(3):433–439.
-  Naraghi, T.S., Emam, M., Ghamrizare, A., Damizadeh, Gh. and Shariat, A., 2013. In vitro propagation of Capparis decidua through shoot tip culture of seedlings and mature trees.Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 20 (1). 134-144.
-  Nazari, A., Delfan, B., Shirkhani, Y., Kiyanei, A.A. and Mandegary, A., 2005. Effect of decoction of Satureja khuzestanica Jamzad on blood coagulation time, triglyceride and glucose levels in rats. Pakistan Journal of Biological Sciences, 8 (6): 790–792.
-  Omrani Sabbaghi, S., Tabaei Aghdaie, S.R., Emam, M. and Bakhshi Khaniki, G.R., 2016. Micropropagation of rare endemic Satureja edmondi.Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(1): 134-141.
-  Sadeghi-Nejad, B., Saki, J., Khademvatan, S. and Nanaei, S., 2011. In vitro antileishmanial activity of the medicinal plant – Satureja khuzestanica Jamzad. Journal of Medicinal Plants Research, 5 (24): 5912–5915.
-  Shahab, A., Haghighati, F., Baeeri, M., Jamalifar, H. and Abdollahi, M., 2011. A clinical, microbiological and immunological comparison between subgingival irrigation with DentolTM and chlorhexidine in advanced periodontitis. Archives of Medical Science, 7 (1): 154–160.
-  Teshome, S., Soromessa, T., 2015. In Vitro Propagation of Satureja Abyssinica (Benth) Briq. A Valuable Medicinal Plant. Advances in Life Science and Technology, 34: 100-109
-  Vezvaei, A., Alamdari, M., Sanei Shariat Panahi, M. and Kashani, M., 2003. Effect of different growing media and plant growth substances on vegetative characters and off shoot production of “Barhi” date palm liners derived from micropropagation. Iranian Journal Agriculture Science. 34 (4): 969-976.
-  Zibaei, M., Sarlak, A., Delfan, B., Ezatpour, B. and Azargoon, A., 2012. Scolicidal effects of Olea europaea and Satureja khuzestanica extracts on protoscolices of hydatid cysts. The Korean Journal of Parasitology. 50: 53–55.
 
 

Files

Share

How to cite

Statistics