Effect of culture media and growth regulators on micropropagation of medicinal plant Urtica dioica L.

Authors

1 Corresponding author, Ph.D. Student, Department of Biology, Payame Noor University, Tehran, I.R. Iran E-mail: sedaghati@rifr-ac.ir

2 Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran

3 Assoc. Prof. Department of Agricultural Biotechnology, Payame Noor University, Tehran, I.R. Iran

4 Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran

5 Assis. Prof. Department of Biology, Payame Noor University, Tehran, I.R. Iran

Abstract

Urtica dioica L. is dioecious plant and cross pollination with active components and special medicinal properties with economic values. Due to high level of heterozygosity of the species, homogeneity in fiber production and harvesting of selected plants is not guaranteed by seed propagation. For this reason micropropagation and asexual proliferation is suggested for production of selected plants. For micropropagation of the species, apical buds of Nettle shoots were used as explant. After sterilization, the explants were transferred into MS medium containing BAP, 2iP and IBA. Then, to evaluate the culture conditions, the explants were located on culturing mediums (MS, DKW, WPM) with different concentrations of growth regulators (IBA, 2,4-D, BAP, KIN, 2iP , TDZ) in a factorial experimental model based on a completely randomized design with three replications. The best medium for shooting initiation was WPM culture. Between rooting treatments, the best root number and secondary roots belonged to KIN (0.5 mgl-1) + IBA (0.5 and 1 mgl-1) and BAP (0.5 mgl-1) + IBA (0.5 mgl-1) treatments, respectively. Then rooted plantlets transferred to the greenhouse and successfully were acclimatized (55.5%).

Keywords

References

-  Ammarellou, A., Kazemeitabar, K., Najafei, H. Z., Mortazavei, N., Ammarellou, N., 2012. Effects of different culture media on rooting of Urtica dioica L. stem cuttings, Journal of Soil Science and Environmental Management, 3(7): 172-175.
-  Darroudi, H., Akbarinia, M., Safarnejad, A., Hosseini, S.M. and Hajian Shahri, M., 2015. Micropropagation of Ribes khorasanicum species by tissue culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research (in Persian), 23(1):65 -76
-  Driver, J.A. and Kuniyuki, A.H., 1984. In vitro propagation of Paradox walnut (Juglans hindsii × Juglans regia ) rootstock. Hort. Science, 19: 507-509.
-  Di Virgilio, N., G.Papazoglou, E., Jankauskiene, Z., Di Lonardo, S., Praczyk, M., Wielgusz, K., 2015. The potential of stinging nettle (Urtica dioica L.) as a crop with multiple uses Industrial Crops and Products,  68: 42-49
-  Gatti, E., Di Virgilio, N., Baronti, S., Bacci, L., 2008. Development of Urtica dioica L. Propagation Methods for Organic Production of Fiber, 16th IFOAM Organic World Congress, Modena, Italy, June 16-20. Archived at http://orgprints.org/view/projects/conference.html
-  Haghpanah, M., Kazemitabar, S. K., Hashemi, S.H.R. and Alavi, S.M. 2014. Evaluation of Mazandaran nettle (Urtica Dioica) population structure and genetic diversity by AFLP markers, Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Vol. 22, No. 2
-  Hedaiat, M. and Khoshkhoii, M., 2008. Effects of media and growth regulators on micropropagation of Pyrethrum, Iranian Society for Horticultural Science, 6:4:171-182
-  Hegi, G., 1981. Illustrierte Flora von Mitteleuropa. III, part 1. 3rd ed. Hamburg: Paul Parey. Journal of Soil Science and Environmental Management Vol. 3(7), pp. 172-175
-  Layous, L., 2013. Response of Pilea Plant Pilea cadieri for Micropropagation using tissue culture techniques, Journal Ordoni in Agriculture Sciences, 9: 619-629.
-  Lloyd, G. and McCown, B., 1981. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by the use of shoot tip culture. Proceeding of Plant Propagation society, 30: 421-427.
-  Luna,T., 2001. Propagation Protocol for Stinging Nettle, NATIVE PLANTS JOURNAL, 2:2:109-111.
-  Moghimi, Z. and Safarvejad, A., 2014. Assessment of micropropagation and flavonoid content of hawthorn through tissue culture, Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research (in Persian), 22: 182-191.
-  Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with tissue cultures. Physiology Plantarum, 15: 473–497.
-  Omrani Sabbaghi, O., Tabaei Aghdaie, S.R., Emam, M. and Bakhshi Khaniki, G.R., 2016. Micropropagation of rare endemic Satureja edmondi, Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(1):134-146.
-  Rahman, M.M., Ankhi, U.R. and Biswas, A., 2013. Micropropagation of Mentha viridis L.: An aromatic medicinal plant, International Journal Of Pharmacy  and Life Sciences, 4(9):2926-2930.
-  Saadat, Y.A., Rasti, O. and Zamani, J., 2012. Effects of different growth regulators, nutrient media, gelling agents and carbohydrate sources on shoot multiplication of Pyrus glabra Boiss. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 20: 84-96.
-  Saeedi Heidari, A. and Safarnejad, A., 2015. Micropropagation of Acer monospessulanum through tissue culture, Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research (in Persian), 23(2): 237-246
-  Szewczuk, M. and Mazur, C., 2004. Effect of different rates of nitrogen fertiliser on chemical composition of Stinging nettle (Urtica dioica L.) Plants Harvested at Three Development Stages Parts I. Contents of Organic Substances, Acta Sci. Pol. Agric. 3(1): 229–237.
-  Zargari A., 1997. Medicinal Plants. Vol. 4, 6st ed., Tehran University Publication, Tehran, P: 103.

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