Evaluation of SO6 protein in Saponaria officinalis L. callus

Authors

1 Corresponding author, Associ. Prof., Biology Science and Technology Center, Imam Hossein University, Tehran, I.R. Iran. Email: Honari.hosein@gmail.com

2 PhD Student of nanobiotechnology, Biology Science and Technology Center, Imam Hossein University, Tehran, I.R. Iran

3 M.Sc., Biotechnology, Islamic Azad University, Science and Research Branch, Tehran, I.R. Iran

4 M.Sc. Student of biology, Biology Science and Technology Center, Imam Hossein University, Tehran, I.R. Iran

Abstract

 Soapwort (Saponaria officinalis L.) is a native plant to central and southern Europe. It is an
important and rare plant species in Iran. The species has numerous applications in medicinal,
agricultural and health fields. Saponins is one of the bioactive compounds in the species that is
used in chemotherapy. Tissue culture is one of the ways of proliferation of the species that is
used for production of active ingredients. Saponin has ten isoforms that are different to
inhibition of translating ribosomes and cytotoxicity. SO6 is one of the isoforms of saponins.
Presence of SO6 protein in soapwort callus was studied through plant tissue culture and
different concentrations of growth regulators and environmental factors. After achieving success
in tissue culture,  protein was extracted from its callus and presence of SO6
protein was confirmed by western blot analysis and measured by ELISA. Results showed that seed
germination was possible through using appropriate treatment and soapwort root callus had the
highest amount of SO6 protein.

Keywords

References

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