Vegetative Propagation of Adult Beech Tree (Fagus Orientalis Lipsky.)

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Research Institute of Forests and Ranglands, Tehran, Iran

Abstract

The main goal of this project is to preserve genetic resources of forests by vegetative propagation of adult trees. Fagus orientalis Lipsky, which its micropropagation, investigated in this project, is one of the most important trees in the forests of Iran. Buds were taken from 3 different genotypes grown in the north forests of Iran. Surface sterilization of the explants was very difficult. Elimination of first three layers of the bud scales was necessary. Intact bud scales caused severe contamination even with very strong sterilizing treatments. Using %0.1 (w/v) solutions of mercuric chloride for 3 minutes was the most effective way for surface sterilization of the explants. The rate of shoot and bud development was very low, and this was the same on WPM and DKW. Culturing established buds horizontally, cold treatment or high auxin concentration pretreatment activated axillary buds. Using 0.2mg/l 2ip (6-dimethylamino purin) together with 0.5 mg/l BA (benzylamino purin) improved shoot proliferation. There were no significant differences between the three genotypes. None of the micropropagated shoots were rooted.

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