In order to investigate in vitro culture of Taxus baccata, apical buds were cut in autumn from selected mature trees at Astara, Asalem forest in Gillan and Aliabadkatol in Golestan province, Iran. Application of 0.1% mercuric chloride solution for 10 minutes was the most effective way for the surface sterilization of the buds. Then the buds were cultured on MS (N/2), B5 and MCM medium plus BA (0.5- 1 mg/L) + IBA 0.1 mg/L. The best treatment for shoot proliferation and elongation was MS (N/2) medium containing 0.5 mg/L BA+ 0.1 mg/L IBA. The shoots were treated as cuttings by dipping the basal ends in 50 mg/L IBA solution for 24 hours, then they were placed in an auxin- free medium plus 1% activated charcoal, for stimulating rooted shoot propagation. Rooted plantlets after acclimation were transferred to mixed soil with equal parts of (vermiculate, sand and peat) under greenhouse conditions.
Naraghi, T. S. (2003). In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 11(3), 309-325. doi: 10.22092/ijrfpbgr.2003.116091
MLA
T. S. Naraghi. "In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture". Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 11, 3, 2003, 309-325. doi: 10.22092/ijrfpbgr.2003.116091
HARVARD
Naraghi, T. S. (2003). 'In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture', Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 11(3), pp. 309-325. doi: 10.22092/ijrfpbgr.2003.116091
VANCOUVER
Naraghi, T. S. In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 2003; 11(3): 309-325. doi: 10.22092/ijrfpbgr.2003.116091
)