The effect of chilling temperatare on peroxidase in nine annual medics species (Medicago spp.)

Authors

.

Abstract

Peroxidase is an indicator enzyme for temperature stress in plant species. The chilling temperature showed increasing rate of peroxidase in annual Medics. Peroxidase has an important role in biochemistry and polymerization (e.g. Lignification) in plant species.
This work was carried out to show the effects of different temperatures 0, 5, 10, 15, 20˚C and green-house conditions on the level of peroxidase enzyme in annual medics. Nine genotypes From six spices of annual medic were established in an aquaculture system at 20˚C for 35 days in a growth-chamber. They were planted in Hogland solution. After 44 days, temperature treatments in a split plot experimental design, the amount of peroxidase of plant shoots were measured.
There was a signifucaut difference of peroxidase level between 0 and 5˚C and the other temperature treatments (10, 15, 20˚C and green house). Therefore, when the environmental temperature decreased the level of peroxidase would increase in annual medic’s shoots. The level of peroxidase as well as peroxidase behavior in relation with temperature treatments varied in different annual medic species.

Keywords

References

سندگل،  ع. و ملک‌پور ب.  1373. مروری بر تحقیقات انجام شده و در حال اجرا در رابطه با یونجه‌‌‌های یکساله در‌ایران و تدوین برنامه کار برای‌ آینده. مؤسسه تحقیقات جنگلها و مراتع،  شماره انتشار 103،  22 صفحه.

Crawford, E.J., 1983. Selecting cultivars from naturally occurring genotypes, Evaluating annual Medicago species. In: MC Ivor, J.G. Genetic Resources of Forage Plants. Melbourne, Australia: CSIRO, 203-215.
Castillo, F.J. 1986. Extracellular peroxidase as marker of stress. In: Molecular and physiological of plant of peroxidase. P.20, University of Geneva, Switzerland.
De Haan, R.L. and Barnes D.K. 1998. Inheritance of pod type stems color, and dwarf growth habit in Medicago polymorpha. Crop Science, 38: 1558-1561.
Ebermann, R. 1975. Bestimmung der Molekulargewichtsverteilung in native starken durch gel chromatography. Strake, 329-333.
Graham, D., and B.D. Pattrson. 1982. Responses of olantsto low, non freezing temperature; proteins, metabolism and acclimation Annu. Rev. Plant Physoil. 33:347-372.
Ghamari Zare, A 1996. Anther culture in intergeneric hybrid of forage grasses. Ph.D. theses. The University of Liverpool, Liverpool, UK, pp.313.
Hanson, C.H. and Barnes D.K. 1988. Alfalfa. In: M.E. Heath, D.S. Metacalf and R.F. Barnes (eds). Forages, The Science of Grassland Agriculture, The lowest. Univ. Press, Ames, Iowa, pp. 136-137.
Kammerer, S. 1993. Seasonal changes of peroxidase isoenzymes and activity of fraction containing plants.
Korori, S.A.A. 1992. Die enzymatische Antwort der Bauwe auf umveltveranderungen am Beispiel Von peroxidase und Katalase Libensmittel &n Biotechnologie, 5:226-229.
Korori, S.A.A., Hinterstoisser B., Long M. and Ebermann, R. 1992. Seasonal alteration of plant peroxidase isoenzymess pattern in Larix decidua. Vol. 32:307-313.
Kuroda, H. Sagiska, Asada S.M. and Chilba, K. 1991. Peroxide-scavenging system during cold acclimation of apple callus in culture. Plant Cell Physiol., 32:635-647.
Levitt, J. 1980. Measurment of drought avoidance. In Responses of Plant Environmental Stresses, Vol. 2. Academic, New York.
Mc Dogall, G.J. 1992. Plant peroxidase and cell differential. Plant Peroxides, 180-190.
Moynihan, J.M., Simons, S.R. and Co Sheaffer C. 1996. Inter-cropping annul medic with confessional height and semi-dwarf barley grown for grain. Agronomy Journal, 88: 823-828.
Siegel, S.M. and Castillo, B.Z. 1986. Peroxidase activity and stress: a complex relationship. In: H Greppin. Molecular and Physiological Aspect of Plant Peroxidase. Univ. Geneva., 427-432.
Van Hugstec R.B. and Esnault, R. 1992. Structure and biosynthesis of peraxidase from peanut cell. Plant Peroxidase, 1986-1996.
Welinder, K.G. 1994. Plant Peroxidase: Structure-Function relationships. Plant Proxidase, 1980-1990.
 

Files

Share

How to cite

Statistics