In vitro propagation of Thuja orientalis by shoot tip culture

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Abstract

Thuja orientalis is an important forest tree species for timber and wood production. Its wood is light in weight and highly resistant to decay. Regarding difficulties in conventional propagation of Conifers, specifically hard rooting of cutting in adult trees this study was carried out.
For investigation of asexual regeneration in Thuja orientalis apical buds from adult trees and seedlings were collected in 3 seasons. After pre- sterilization, the best method for sterilizing of buds was identified. The method was: Cleaning and brushing the buds by tween and ethanol 70% solution as the pretreatment, dipping them in ethanole 70% for 1 minutes and cleaning buds with 0.1% HgCl2  solution in different periods (4 for spring, 6 for summer, and 9 minutes for fall). The suitable medium for establishment was DKW medium with 0.5 mg/lit 2iP. Established explants were subcultured in 3 media (MS, DKW and SH) containing 2iP and BA alone or in combination (from 0 to 0.5 mg/lit) + IBA (0.01 mg/lit). After  two subculturing of buds in the media, the means of shoot proliferation and shoot length growth was used as the data for analysis of varience. The best medium for shoot regeneration was the DKW with 0.5 mg/lit 2iP, 0.01 mg/lit IBA(83%). Rhyzogenese of shoots was done in DKW medium supplimented with 2 mg/lit IBA (33%) .

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