Influence of Mediume, Hormone and Genotype on Micropropagation of Populus Capsica

Authors

1 Faculty Member of Forestry and Rangeland Research Center

2 Member of faculty at Tarbiat Moalem University

3 Member of faculty of forest and rangeland research institute

Abstract

Shoot tips from different genotypes of Populus caspica, were collected from adult trees grown in northern forests of Iran. After surface sterilization, these explants were cultured on three different media containing BA (0.5 - 2) mg/lit and IBA (0.01) mg/lit .The rate of shoot multiplication, shoot length growth and other responses of these genotypes were investigated. All of them showed rapid multiplication, when cultured on half strength MS containing 0.5 - 2 mg/lit BA and 0.01 mg/lit IBA. The number of shoots formed per explants varied for each genotype. Genotype 3 had the highest proliferation in the same conditions. All of such shoots rooted in MS medium with 0.5 mg/lit IBA. This plantlets were transferred in soil and 100 % of these plants successfully established in the field conditions.