Micropropagation of Populus Euphratica Using Tissue Culture

Authors

1 Forestry and Rangeland Research Institute

2 Faculty Member of Forestry and Rangeland Research Center

Abstract

After selecting the elite trees of Populus euphratica in different localities, shoot tips were excised and the buds were sterilized with different treatments as follows:
1 - Washing by tap water and detergent.
2 - Sterilizing in 1% benlate for 30 to 60 minutes.
3 - Sterilizing in 70% ethanol for 5 minutes.
4- Sterilizing in 0.1% mercuric chloride for 1 minute.
These werel selected as the best methods of sterilization. The explants were established in different media with different ranges of hormones. Regarding factors such as shoot length growth, roliferation, greenish, callus induction and vitrificated explants in this experiment the best medium for establishing, shooting and regeneration of shoots was selected. MS medium with half strength of nitrate and BA: 0.5 mg/l, Kinetin:0.5 mg/l, IBA: 0.01 mg/l were selected as the best medium. Then rhyzogenese treatments on this media were done with changing concentration, type and combination of rooting hormones. The best rooting level was obtained by 0.1 mg/l of IBA. Then the plantlets were established in strile soil (Peat - Sand - Homus in equal perecentages) with polyethylen cap and this plantlets then were transferred to germinator. Acclimatization steps on this plants were successfully accomplished.

Files

Share

How to cite

Statistics