Somatic embryogenesis in some Eucalyptus spp.

Author

Nanral Resources Livestock Affair Research center of khoozestan hovince, Ahvaz, Iran

Abstract

Induction of somatic embryogenesis was attempted in this study. Explants type (anthers, hypocotyls, cotyledons, leaf discs and petioles), media type (B5 and MS); auxins (NAA and 2,4-D) and cytokinins (TDZ, BAP) as well as environmental conditions (light and dark, temperature) were evaluated. Embryogenic callus of E. camaldulensis originating from hypocotyl explants was formed on the primary medium and developed on the secondary .medium' when specific hormonal formulation was used. A large number of synchronous embryos germinated and matured in the light. Without growth regulators. Secondary embryos were also obtained on solid Inedia. E. sargentii produced some asynchronous embryos. When the same method was used for somatic embry Digenesis of other Eucalyptus spp. rhizogenesis, or organogenesis, resulted, but no embryogenesis. Attempts to produce embryogenic suspension cultures were unsuccessful. Plantlets produced from somatic embryos were successfully weaned and grown in the greenhouse for several months and their appearance was morphologically normal.

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