Somatic embryogenesis in leaf culture of Populus deltoides Bartr.ex Marshreastern

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Department of Genetics and Plant Physiology, Research Institute of Forests anci Rangelands, P.O. Box 13185-116, Tehran, Iran

Abstract

In order to develop somatic embryogenesis for genetic manipulation process of Populus deltoides Bartrex Marsh eastern, isolated leaf disk explants were cultured on MS medium supplemented with 2,4-D (2,4-dichlorophenoxyacetic acid), BA(benzyladenine) and glutamine. High number of somatic embryos formed on both the midveins and secondary veins. MS medium containing 2 mg/l 2,4-D and 0.1 mg/l BA with 20 J.LM glutamine showed highest number of globular-shaped embryo (11.6). Most of the embryogenic calli were formed on the cut edges of the leaf disks in MS medium containing 5 mg/12,4-D and 0.1 mg/1 BA for all glutamine concentrations. MS plant regeneration media supplemented with 2 mg/l BA and 0.1 mg/1 NAA (Naphtalene Accetic Acid) produced high percentage (72.5%) of plantlets on callused leaf disks explants. Rooted plantlets in WPM basal medium were successfully acclimatized on soil medium (1:1:1 peat, perlit and vermiculite) and transferred to the field.

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